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6.3.2 In vitro flowering
In vitro corm formation and flowering and ex vitro acclimatization
Although an in vitro formed flower was observed for R. minutiflora during corm
formation experiments at 20°C on a medium with 9% sucrose (Figure 6.1), none of
the further experiments yielded any flowers or flower initials.
Figure 6. 1: An in vitro formed flower of Romulea minutiflora observed in a test tube placed at
20°C on a medium with 9% sucrose.
6.3.3 Ex vitro acclimatization and corm viability
None of the corms or plantlets survived more than one month. Four corms planted
inside the plastic container however survived (Fig. 6.2) for two months, and 7 out of
10 corms tested were viable.
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