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In vitro culture initiation and multiplication
There was a significant difference between the number of shoots produced on media
supplemented with 2.5 M mTR and the number of shoots produced on media
supplemented with all the kinetin concentrations tested including 2.5 M kinetin
(Figure 5.9). Although the highest number of shoots were also produced on media
supplemented with 2.5 M mTR, there was no significant difference between the
number of shoots produced on this medium and the number of shoots produced on a
medium supplemented with 2.5, 5.0 or 7.5 M BA, 5.0 or 7.5 M mTR, or 2.5 or 5.0
MemTR.
The shoots produced on a medium supplemented with 2.5 M mTR appeared
healthy and 40% of them were rooted. All other media treatments also produced
healthy shoots of which at least 20% were rooted, except for media supplemented
with BA, on which vitrified shoots were observed as with previous experiments.
Average number of shoots per explant
8
6
4
2
0
cd
Control
d
2.5 µM kinetin
cd
5.0 µM kinetin
bcd
7.5 µM kinetin
ab
2.5 µM BA
abcd
5.0 µM BA
abc
7.5 µM BA
bcd
2.5 µM mT
bcd
5.0 µM mT
cd
7.5 µM mT
a
2.5 µM mTR
Media treatments
abcd
5.0 µM mTR
abcd abc
Figure 5.9: Effect of three different concentrations of five cytokinins on multiplication of
Romulea sabulosa shoots after 2 months. Error bars indicate standard error of the mean.
Letters shows significant differences between treatments according to Duncan’s multiple
range test.
7.5 µM mTR
2.5 µM MemTR
abcd
5.0 µM MemTR
bcd
7.5 µM MemTR
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