Self-assembled Transition Metal Coordination Frameworks of ...

Chapter 2
Table 2.8. Hydrogen-bonding geometry of HZL4.
D—H---A D—H (A) H---A (A) D---A (A) D—H---A (°)
N(4)—H(lN4)---N(1) 0.92 (2) 2.03 (2) 2.707 (2) 130 (2)
N(4)—H(lN4)---N(6) 0.92 (2) 2.25 (2) 2.613 (2) 103 (1)
N(5)—H(lN5)---N(8_) 0.87 (2) 2.04 (2) 2.702 (2) 132(2)
C(2)—H(2A)---0(1)‘ 0.93 2.55 3.213 (2) 128
C(4)—H(4A)---N(2) _ 0.93 2.60 2.982(2) 105
C(16)—H(l6A)---0(1)‘ __ 0.93 2.53 3.348 (2) 146
c(1 l)—H(1 IA)---Cg(4)“ 0.93 2.78 3.466 (2) 131
D= donor, A=acceptor; Symmetry codes: (i) x+l, y, z (ii) x, y, 2+1.
2.4. Anticancer studies
Anticancer activity (in vitro) of compounds was analyzed by standard growth
inhibitory assays. Mechanism of action is analyzed in MCF-7 (Michigan Cancer
Foundation-7) cell lines and is one of the three cell line panel recormnended by NCI
(National Cancer Institute) for anticancer drug screening (NCI developmental
therapeutic program). Determinations of the effectiveness of the compounds were
achieved by MTT Cell Proliferation Assay.
2.4.1. Maintenance of cell line
The healthy MCF-7 cells were seeded in the T 25 cmz tissue culture flask with
complete media i.e. DMEM (Dulbecco’s Modified Eagle Medium) (Sigma), 10% FBS
(Foetal Bovine Serum), with antibiotics (100 mg/ml of pencillin, 100 mg/ml
streptomycin) and allowed to become confluent. When cells were grown to
confluency, the medium was removed, and washed once with PBS (phosphate
buffered saline). 0.25% Trypsin-EDTA solution was added, and incubated for 5-10
minutes at 37 °C. Fresh medium (with serum) was added and a pipette gently
dispersed cells. A known number of cells were dispensed in to new flasks or
microtitre plates for further experiments. The cells were incubated at 37 °C and 5%
CO2 atmosphere.
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