Self-assembled Transition Metal Coordination Frameworks of ...

Chapter 2
0 The compounds were then diluted in DMEM with 2.5% Fetal Bovine Serum.
~ The drug-containing medium (100 ul of media with known concentrations of
each compound) was added.
~ The cells were incubated at 37 °C / 5% CO2 for 48 h.
v Six wells, containing cells grown in drug-free medium were kept to determine
the control cell survival and the percentage of live cells after culture.
~ After incubation, the drug-containing medium was removed and 100 ul of
21% MTT (5 mg/ml) solution in 2.5% DMEM was added to each of the wells.
~ The plates were kept for another 2‘/2 h for incubation at 37 °C / 5% CO2.
- The yellowish colored MTT was reduced to dark-colored formazan by viable
cells only.
~ Then, 100 pl of MTT lysis buffer (a mixture of 20% SDS in 50% DMF) was
added to solubilize the formazan crystals.
0 The color developed was quantified using an ELISA plate reader at 570 nm.
~ Cell survival expressed as percentage and was calculated as:
Cell survival (CS) = (Mean OD d,ug_t,¢,,¢dc¢||, / Mean OD ¢°,,m,|ce||,) X 100
A graph is plotted taking the concentration of the drug (X-axis) against the
percentage viability (Y-axis). LD50 value (lethal dose of drug for killing 50% of cells)
of the drug indicates the effectiveness of the compounds used; the lesser the value, the
more effective the compounds are.
The ligands I-l2L', HZL3, HZL4, H2L° and HL7 were independently tested using
the above procedure towards MCF-7 cell lines for their unique features and the results
are summarized in Table 2.9 and Fig. 2.21. It is seen that the thiocarbohydrazones
(H2L' and H2L3) and carbohydrazones (HZL4 and H2L°) show proliferation effect.
However, the thiosemicarbazone HL7 shows antiproliferation effect.
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