14th ICID - Poster Abstracts - International Society for Infectious ...

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When citing these abstracts please use the following reference: Author(s) of abstract. Title of abstract [abstract]. Int J Infect Dis 2010;14S1: Abstract number. Please note that the official publication of the International Journal of Infectious Diseases 2010, Volume 14, Supplement 1 is available electronically on http://www.sciencedirect.com Final Abstract Number: 28.016 Session: Influenza Date: Wednesday, March 10, 2010 Time: 12:30-13:30 Room: Poster & Exhibition Area/Ground Level Type: Poster Presentation Development of a consensus microarray method for identification of different influenza A viruses D. Barlic - Maganja 1 , A. Fratnik Steyer 2 , N. Toplak 3 , A. Zrimec 4 , O. Zorman Rojs 2 1 University of Primorska, Izola, Slovenia, 2 University of Ljubljana, Ljubljana, Slovenia, 3 OMEGA Ltd., Ljubljana, Slovenia, 4 Institute of Physical Biology Ltd., Ljubljana, Slovenia Background: Rapid and reliable identification methods are fundamental for the comprehensive characterization of emerging and evolving influenza viruses. A consensus microarray approach for detection and a panel of specific probes covering 16 hemagglutinin (HA) subtypes of influenza type A viruses has been designed. Methods: Nucleotide sequences of all available influenza A virus subtype combinations, isolated from various species on different geographic regions were selected from GenBank and from influenza virus specific databases (FLU Database, Influenza Research Database). Among them 192 sequences of matrix (M) protein gene and 206 sequences of hemagglutinin (HA) gene were aligned and arranged, respectively, using ClustalX and BioEdit programmes. Results: For the influenza A virus detection oligonucleotide probes were selected on the virus M gene. 16 different segments of this gene were used for the construction of 37 probes. The probes for simultaneous identification of different subtypes were selected on 6 different parts of the virus HA gene. For each HA subtype at least 10 probes were designed on the 1770 nts long segment 4. Conclusion: Oligonucleotide microarray method was designed for simultaneous detection and subtyping of influenza A viruses. The evaluation of developed approach including all 16 HA subtypes is in progress.
When citing these abstracts please use the following reference: Author(s) of abstract. Title of abstract [abstract]. Int J Infect Dis 2010;14S1: Abstract number. Please note that the official publication of the International Journal of Infectious Diseases 2010, Volume 14, Supplement 1 is available electronically on http://www.sciencedirect.com Final Abstract Number: 28.017 Session: Influenza Date: Wednesday, March 10, 2010 Time: 12:30-13:30 Room: Poster & Exhibition Area/Ground Level Type: Poster Presentation Clinic-epidemiologic identification of the first wave of AH1N1 in adults in Albania N. Como 1 , D. Kraja 2 , P. Pipero 3 , S. Bino 4 , E. Meta 3 , N. Gjermeni 3 , E. Muco 3 , A. Kica 3 , E. Erindi 4 , V. Ostreni 3 , R. Petrela 1 , S. Cibuku 3 1 University Hospital Centre "Mother Theresa", Tirana, AL, Albania, 2 Faculty of Medicine, Tirane, Albania, 3 HUC, Tirane, Albania, 4 Institute of Public Health, Tirane, Albania Background: Our goal is the recognition of demonstration clinical-epidemiologic of AH1N1 first wave.This material includes 32 cases of ages 15 – 66 years old,during June-September 2009.The influenza identification is based on criteria of WHO. Methods: The cases were classified based on chronology demonstration, origin, group-age and gender.There were analyzed symptoms with what influenza was represented;it was conceived its clinical syndrome based on the subject.There were evidenced and complications. Results: Epidemiological aspects: Chronological:June 9,July 5,August 17,September 1 case. Imported cases 27(England 12,Greece 7,Italy 6,Macedonia 1,Malt 1);autochton 5. Based on group-age Age in years Number of cases 15-24 10 25-44 13 45-59 6 60-66 3 Where 20 were male and 12 female. Clinical aspects: General syndrome appeared with subfebrile temperature Temperature Number of Cases 37.60C - 37.90C 2 38.20C- 39.40C 30 with hyperpyrexia 0 The fever extended: Number of days Number of cases 1 18 2 9 3 3 4 1 5 1 Symptomps Number of cases sweating 32 general myalgia 13 lower extremitetes myalgia 9 headache 29 diarrhea 6 vomiting 7 loss of appetite 25 weakness 27 Local syndrome was presented as 1)Rhinitis (nose congestion 14, exudative rhinorrhea 15, sneezing 17) 2)Pharingitis 18 cases (dryness and sore throat 18, pain of pharynx 13) 3)Laryngitis 3 cases with hoarseness and 1 with dyspnea; 4)Catarrhal angina 19 cases; 5)Tracheitis 17 cases (cough 17, sputum 7, chest pain 10) 6)Bronchitis 13 cases(unproductive cough 13,productive cough 9;chest pain 7,dyspnea 2,bronchial rale 13) 7)Tracheo-bronchitis 9 cases 8)Bronchiolitis 5 cases 14 cases were healthy subjects;7 cases had chronic diseases (diabetes mellitus 4,cirrhosis 1, cholagenosis 1,lymphoma 1);1 was 4 months pregnant. Complications: Diagnosis Number of cases Otitis 1 Myositis 1 Encephalopathy 2 Pneumonia 8 ARDS 4 cases(2 with multiorganory failure) Conclusion: 1.AH1N1 first wave extended June- September; the maximum reached on August with 53.12% of cases. 2. Cases were imported from 5 countries; majority 37.5% from England. 3.Affected age fluctuated from 15-66 years old; predominated group-age 25-44 years old, 40.62%. 4.The temperature fluctuated from 37.6- 39.4 0C; 6.25% were subfebrile. 5.There were highlighted 7 clinical syndromes and 6 cases of complications.
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