14th ICID - Poster Abstracts - International Society for Infectious ...

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When citing these abstracts please use the following reference: Author(s) of abstract. Title of abstract [abstract]. Int J Infect Dis 2010;14S1: Abstract number. Please note that the official publication of the International Journal of Infectious Diseases 2010, Volume 14, Supplement 1 is available electronically on http://www.sciencedirect.com Final Abstract Number: 34.024 Session: Zoonoses and Infectoins in Animals Date: Wednesday, March 10, 2010 Time: 12:30-13:30 Room: Poster & Exhibition Area/Ground Level Type: Poster Presentation Epidemiology of avian influenza viruses in wild birds in Mongolia E.-O. Tseren-Ochir 1 , B. Damdinjav 1 , T. Sharkhuu 1 , H. M. Kang 2 , Y. Sakoda 3 , B. Purevsuren 4 , S. Ruuragchaa 1 , Y. J. Lee 2 , H. Kida 3 , B. Khishgee 1 , S. Sengee 1 1 State Central Veterinary Laboratoy, Ulaanbaatar, Mongolia, 2 National Veterinary Research & Quarantine Service, Anyang-city, Korea, Republic of, 3 Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan, 4 Avian influenza and human pandemic influenza preparedness and response project of World Bank, Ulaanbaatar, Mongolia Background: A combination of geographical features makes Mongolia an ideal location for understanding the epidemiology of avian influenza viruses in wild birds. There are four main migration routes (East-Asia/Australasia, Central-Asia/India, West-Asia/Africa and Mediterranean/Black-see) passing through Mongolia. Around 391 species of migratory birds arrive in Mongolia. Mongolia also represents an important site for molting of Anseriformes. Methods: Since 2006, we have collected 3950 feces samples and 150 tissue samples from wild birds during the active surveillance in Mongolia. Viruses isolated by the egg inoculation method, and RT-PCR, qRT-PCR and Neuraminidase inhibition test were used for subtyping. The confirmation of the isolated highly pathogenic avian influenza virus(HPAIV), and intravenous pathogenicity index(IVPI), phylogenetic analysis were performed by the OIE Reference Laboratory for HPAI at Hokkaido University, Japan. The confirmation of the low pathogenic avian influenza virus (LPAIV) isolates and phylogenetic analysis were carried out in the National Veterinary Research and Quarantine Service of Korea. Results: In 2005 and 2006, 2 HPAIV(H5N1) were isolated from wild birds (whooper-swan, barheaded-goose and common-goldeneye) at Erkhel and Khunt Lakes. These viruses were classified into the clade 2.2 so-called Qinghai Lake-type. In May and August, 2009, 2 HPAIV(H5N1) were isolated from wild birds (whooper-swan and barheaded goose) at Doitiin tsagaan and Duruu Lakes. These viruses were classified into the clade 2.3.2. The IVPI was high /2.97-3.00/. Also we isolated 21 LPAIVs in 2007 and 2008. The subtypes were H3N8(11), H4N6(4), H7N7, H7N9, H3N1, H3N2, H4N2 and H10N6. The viruses were related with the Euro-Asia lineage. In 2009, 7 LPAIVs were isolated from wild birds (whooper-swan, ruddy-shelduck, Mongolian-gull, mallard and gadwall) in Central and Eastern Provinces. The subtypes were H3N8(3), H10N6(3) and H4N6. Conclusion: We isolated 4 HPAIVs and 28 LPAIVs from wild birds in Mongolia genetically related to the Euro-Asian AIVs. All HPAI outbreaks were restricted to the wild birds in northcentral Mongolia. The phylogenetic differences of the H5N1 isolates from 2005, 2006 and 2009 indicate that the role of the migratory birds in Mongolia in the AIV mutation should be clarified. Therefore, it is necessary to continue the research on avian influenza in Mongolia.
When citing these abstracts please use the following reference: Author(s) of abstract. Title of abstract [abstract]. Int J Infect Dis 2010;14S1: Abstract number. Please note that the official publication of the International Journal of Infectious Diseases 2010, Volume 14, Supplement 1 is available electronically on http://www.sciencedirect.com Final Abstract Number: 34.025 Session: Zoonoses and Infectoins in Animals Date: Wednesday, March 10, 2010 Time: 12:30-13:30 Room: Poster & Exhibition Area/Ground Level Type: Poster Presentation Virological studies on migratory penguins captured in Brazilian southeast coast L. A. Fornells Arentz 1 , I. BIANCHI 2 , C. E. P. F. Travassos 2 , M. H. T. Liberal 3 , C. D. M. Andrade 1 , J. N. S. S. Couceiro 1 1 Federal University of Rio de Janeiro, Cidade Universitária, Brazil, 2 Unversidade do Norte Fluminense - , Campos dos Goytacazes, Brazil, 3 PESAGRO-RJ, Niteroi, Brazil Background: The migratory birds represent the main vector of Orthomixovirus and Paramyxovirus in nature, spread among birds in temperate regions. They have been reported circulating among Antarctic penguins through serological surveys, especially in Adelie penguins (Pysoscelis adeliae) of eastern Antarctica. The Magellanic penguins (Spheniscus magellanicus) are distributed on the southern shores of South America. Their breeding colonies were distributed from the coast of Chile to the Peninsula Valdez in Argentina, on the southern tip of South America. This penguin performs migration to the north, and such shift is an annual event considered characteristic of the species. The birds breed in colonies north of its distribution, as in the southern coast of Brazil, where they stay in the winter. Some individuals have demonstrated irregular movements that may occur with or in large numbers, causing errant individuals. It is believed that for this reason this species have reached the Northeast of Brazil in 2008 and made an unusual situation. Methods: We analyzed the presence of Orthomixovirus and Paramyxoviruses in penguins captured on the coast of the Espírito Santo state, Brazil, due to the increasing number of species in the region in 2008. Were inoculated into the allantoic cavity embryonated fowl eggs suspensions of 73 cloacal swabs taken from live migratory penguins at Brazilian Southeast Coast, between September - October 2008. The allantoic fluids were tested for haemagglutination activity (HA). In samples with positive HA, we performed hemagglutination inhibition (HI) test against antibodies to H1N1, H2N2, H3N8, H7N7, and Newcastle disease virus (NDV). Neuraminidase ativity (NA) assay was performed to characterized the isolates. Were perfomed a RT-PCR to protein M of Influenzavirus and L protein of broadly range of Paramyxovirus. NA activity and inhibition by DANA of penguins isolates. Results: Our results shown that 9 samples (12%), were positive by haemaglutination test, but no inhibition by influenza sera observed. Partial inhibition by NDV serum was observed in all samples. All samples presents NA activity. All samples amplified L protein gene of Paramyxovus (Avulavirus), demonstrating a strong band of 500 bp on agarose gel. Conclusion: Our results shown that Avulavirus is present on these birds and may cause diseases on this species, contributing to clinical deterioration of the animals.
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