14th ICID - Poster Abstracts - International Society for Infectious ...

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When citing these abstracts please use the following reference: Author(s) of abstract. Title of abstract [abstract]. Int J Infect Dis 2010;14S1: Abstract number. Please note that the official publication of the International Journal of Infectious Diseases 2010, Volume 14, Supplement 1 is available electronically on http://www.sciencedirect.com Final Abstract Number: 26.003 Session: Foodborne Diseases and Outbreaks Date: Wednesday, March 10, 2010 Time: 12:30-13:30 Room: Poster & Exhibition Area/Ground Level Type: Poster Presentation Burden of illness for food-borne Salmonella: a cohort study in an agricultural community in Yucatan, Mexico M. B. Zaidi 1 , F. D. Campos 1 , F. Martinez 1 , F. Gutierrez 1 , M. Leon 1 , A. Polanco 1 , T. Estrada- Garcia 2 , J. J. Calva 3 1 Hospital General O'Horan, Merida, Yucatan, Mexico, 2 Centro de Investigacion y Estudios Avanzados del IPN, Mexico City, Mexico, 3 Instituto Nacional de Ciencias Medicas y Nutricion "Salvador Zubiran", Mexico City, Mexico Background: There is a great need for conducting community-based integrated food-chain surveillance in highly endemic settings to assess the impact of Salmonella infections on human health. Methods: A 15-month cohort study was conducted in Buctzotz, a small, well nourished, agricultural community in Yucatan, Mexico. Twice-weekly household visits for surveillance of diarrheal disease, collection of stool samples and health education were performed in 126 infants less than 3 years of age and 120 adults over 74 years. Ten samples each of food-animal intestines, raw retail meat, and ready-to-eat food and beverages were tested weekly. Salmonella isolates were serotyped, and tested for antimicrobial susceptibility and PFGE according to standard methods. Results: Salmonella contamination rates in chicken, swine and cattle intestine and raw retail meat were 20% and 27%; 82% and 69%; and 74% and 75%, respectively. Salmonella was also recovered from 5% of cooked pork, 4% of cooked chicken and 4% of fresh fruit beverages. The annual incidence of diarrhea of any etiology was 2.1 episodes/infant and 0.7 episodes/elder. Incidence of Salmonella-associated diarrhea was 21 episodes/100 infants and 13 episodes/100 elders. Asymptomatic carriage was detected in 18% of household members. None of the cohort subjects suffered dehydration or required hospitalization. The distribution of Salmonella serotypes in humans closely correlated with those isolated from animals and foods. PFGE analysis of the two top serotypes, Agona and Meleagridis, and two virulent serotypes, Enteritidis and Typhimurium, showed that many human isolates were indistinguishable or closely related to animal isolates. In the few household clusters detected, the infant was symptomatic and older family members were usually diarrhea-free. Overall, 3.5% of isolates were resistant to ceftriaxone, 17.7% to nalidixic acid, and 0% to ciprofloxacin; 5.6% were resistant to ampicillin, chloramphenicol and trimethoprim-sulphamethoxazole. Conclusion: In highly endemic settings, multiple clones of Salmonella circulate in the food chain and are continuously transmitted to humans of all ages. Constant exposure results in sporadic symptomatic infections at the extremes of age and asymptomatic infections at other ages. Future preventive interventions should focus on health education aimed at reducing household transmission, and on the rational use of antimicrobials in food animal production.
When citing these abstracts please use the following reference: Author(s) of abstract. Title of abstract [abstract]. Int J Infect Dis 2010;14S1: Abstract number. Please note that the official publication of the International Journal of Infectious Diseases 2010, Volume 14, Supplement 1 is available electronically on http://www.sciencedirect.com Final Abstract Number: 26.004 Session: Foodborne Diseases and Outbreaks Date: Wednesday, March 10, 2010 Time: 12:30-13:30 Room: Poster & Exhibition Area/Ground Level Type: Poster Presentation Laboratory investigation for clonality of a foodborne outbreak due to Vibrio parahaemolyticus in Singapore, 2009 M.-V. La 1 , S. Zulaina 1 , R. Jureen 2 , R. Lin 2 1 National Public Health Laboratory, Singapore, Singapore, 2 National University Hospital, Singapore, Singapore Background: We report a laboratory investigation of a gastroenteritis outbreak caused by Vibrio parahaemolyticus following the consumption of the local salad dish “Indian rojak” from a popular hawker stall in Singapore in April 2009. The total number of involved cases was 154, with 48 cases hospitalized and 2 dead. The National Public Health Laboratory collaborated with the investigation of collected isolates of V. parahaemolyticus to determine genetic relatedness of these isolates. Methods: Repetitive extragenic palindromic PCR (REP-PCR), PCR for the thermostable direct hemolysin gene (tdh) and the tdh-related hemolysin gene (trh) as well as serotyping were performed on all isolates from suspected outbreak cases and some unrelated control strains. The REP-PCR fingerprint was generated with the Agilent® Bioanalyzer using DNA 1000 LabChip® kit, and then analyzed with Bionumerics software. Results: REP-PCR profiles obtained from 15 of 16 investigated isolates were identical. REP- PCR typing appeared to be as discriminatory as pulse-field gel electrophoresis in this outbreak investigation. All above outbreak isolates were positive for tdh, negative for trh and had serotype O4:K55. Conclusion: REP-PCR in this setting was a rapid and useful molecular typing method for the laboratory evaluation of genetic and epidemiological relationships among V. parahaemolyticus strains.
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