14th ICID - Poster Abstracts - International Society for Infectious ...

When citing these abstracts please use the following reference:
Author(s) of abstract. Title of abstract [abstract]. Int J Infect Dis 2010;14S1: Abstract number.
Please note that the official publication of the International Journal of Infectious Diseases 2010, Volume 14, Supplement 1
is available electronically on http://www.sciencedirect.com
Final Abstract Number: 25.019
Session: Clinical Bacterial Infections
Date: Wednesday, March 10, 2010
Time: 12:30-13:30
Room: Poster & Exhibition Area/Ground Level
Type: Poster Presentation
Anaerobic bacteria as etiological agents of intraabdominal infections from a Costa Rican hospital
C. Quesada-Gómez, E. Rodríguez-Cavallini, M. D. M. Gamboa-Coronado
University of Costa Rica, San José, Costa Rica
Background: Intraabdominal infections (IAI) are serious life-threatening conditions that endanger
patient’s life and are caused usually by the indigenous intestinal flora. The study of the anaerobic
bacteria involved in the IAI contributes for a better treatment; nevertheless, it is performed by few
laboratories in Costa Rica as well as other Latin American countries.
Methods: Two hundred thirty one intraabdominal samples were studied by anaerobic bacteria.
Samples were taken by medical staff and sent, under anaerobic conditions, to the Anaerobic
Bacteriology Research Laboratory of the University of Costa Rica. From the San Juan de Dios
Hospital, Costa Rica (for nine years: 1999 to 2008).
Once there, each one was inoculated into prereduced media and incubated for 48 h at 35ºC.
After this, each sample was streaked in blood agar supplemented with vitamin K and hemin in
order to isolate characteristic colonies. These were identified using Gram staining, physical
colonial characteristics, and hemolysis. For definitive identification the RapID 32A®
(bioMérieux) system was used.
Results: A total of 265 anaerobic isolates were obtained from 129 positive samples (56%): 71%
of the strains were Gram positive and 29% Gram negative; 2 isolates per sample. Bacteroides
was the genus most frequently isolated (22%), followed by Eggerthella and Clostridium (16 and
15%), The species of B. fragilis group were the most frequent (more details in the
table)Anaerobic bacteria as etiological agents of intraabdominal infections from a Costa Rican
hospital (1999 – 2008)
Table
Conclusion: Knowledge of the etiological agents involved contributes to the therapeutic
success in IAI; so the study of anaerobic bacteria is helpful, not just in Costa Rican hospitals, but
in those of the Latin American region.