14th ICID - Poster Abstracts - International Society for Infectious ...

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When citing these abstracts please use the following reference: Author(s) of abstract. Title of abstract [abstract]. Int J Infect Dis 2010;14S1: Abstract number. Please note that the official publication of the International Journal of Infectious Diseases 2010, Volume 14, Supplement 1 is available electronically on http://www.sciencedirect.com Final Abstract Number: 23.018 Session: Antibiotic Resistance Gram-Negative Date: Wednesday, March 10, 2010 Time: 12:30-13:30 Room: Poster & Exhibition Area/Ground Level Type: Poster Presentation A Comparative study on gram-negative bacterial infections in Mansoura University Hospitals, Egypt W. El-Naggar, R. Ibrahim, E. Habib, S. Gerorge, E. Abd-Elmagid Faculty of Pharmacy, Mansoura, Egypt Background: Gram negative bacteria are responsible for numerous infectious diseases. These diseases can occur in and harm any part of the body, the skin, eyes and the nervous, cardiovascular, respiratory, gastrointestinal and urogenital systems. Methods: In the present study, some phenotyic and molecular typing techniques were applied on 108 strains of E. coli, 88 strains of Ps. aeruginosa and 8 strains of Serratia isolated from different clinical lesions in Mansoura University Hospitals, Egypt. Results: The distribution of antibiotic resistance among the isolated strains showed high incidence of resistance and imipenem was the most active antibiotic. Using the active pyocin typing, 72 strains of Ps. aeruginosa could be typed into 35 pyotypes. SDS-PAGE of total cell protein extracts showed that the presence of fifteen patterns among E. coli strains and eleven patterns among Ps. aeruginosa strains. Using PCR technique it was found that 84% of the 50 tested strains were found to have at least one of the tested ESBLs. Also TolC and AcrA genes were present in all tested E. coli except 4 strains and did not present in Ps. aeruginosa except 4 strains. Plasmid profiles of 23 tested E. coli appear to be diverse. Also the prevalence of plasmids in 22 tested Ps. aeruginosa strains was lower than in tested E. coli therefore 59.1% of tested Ps. aeruginosa strains harbored plasmids. Using Pyrosequencing technique, the sequenced region of gyrA, gyrB and ParC were able to differentiate between the tested strains and neighbor-joining tree was constracted to determine relatedness between the isolated strains. Morover, Molecular cloning of the whole sequence of bla-TEM, bla-SHV and bla-CTX-M was carried out experimentally to study the expression of these genes and determine which genes of them responsible for the resistance Conclusion: Molecular-based methods of typing are more advantageous compared with phenotypic methods of typing in terms of better discrimination and reproducibility. Significant genetic variation was observed among different strains represented by the diversity of their plasmid profiles. All molecular genetic methods for distinguishing organism subtypes are based on differences in the DNA sequence.
When citing these abstracts please use the following reference: Author(s) of abstract. Title of abstract [abstract]. Int J Infect Dis 2010;14S1: Abstract number. Please note that the official publication of the International Journal of Infectious Diseases 2010, Volume 14, Supplement 1 is available electronically on http://www.sciencedirect.com Final Abstract Number: 23.019 Session: Antibiotic Resistance Gram-Negative Date: Wednesday, March 10, 2010 Time: 12:30-13:30 Room: Poster & Exhibition Area/Ground Level Type: Poster Presentation Extended spectrum beta-lactamases in Escherichia coli and Klebsiella spp. from Eastern Romania E. Miftode 1 , O. Dorneanu 2 , D. Leca 3 , A. Teodor 4 , A. Badescu 5 , G. Juganariu 5 , A. Vita 5 , C. Dorobat 5 1 Univ. of Medicine and Pharmacy - Hospital of Infectious Diseases, Iasi, Romania, 2 Univ Medicine and Pharmacy Iasi, Iasi, Romania, 3 Univ. of Medicine and Pharmacy, Iasi, Romania, 4 Univ of Medicine and Pharmacy, Iasi, Romania, 5 Univ Medicine and Pharmacy, Iasi, Romania Background: The emergence and dissemination of ESBL are problems of major importance for the population health; ESBLs represent a first example of factor that contribute to the global crisis concerning the treatment of Klebsiella pneumoniae and Escherichia coli against which the third generation cephalosporins are not efective anymore. Methods: Clinically isolates of E. coli (n= 642) and Klebsiella (n=92 ) were collected from patients with different types of infections (sepsis, urinary tract infections, etc), hospitalyzed between september 08 and september 09 in a Hospital of Infectious Diseses from Eastern Romania. Double disc synergy test using cefotaxime and amoxicillin/clavulanic acid discs was used to screen ESBL producers and these strains were subsequently subjected to confirmatory Etest. Results: E. coli resistance to cefotaxime, ceftazidime, cefoxitin, ciprofloxacin and imipenem was found to be 40, 29, 6, 30 and 1% respectively. Klebsiella resistance to cefotaxime, ceftazidime, cefoxitin, ciprofloxacin and imipenem was 70, 57, 22, 41 and 4% respectively. % of ESBL producers E. coli was 15% (97 strains) and Klebsiella was 38% (35). All the ESBL producing strains were susceptible in 100% to imipenem and meropenem. Conclusion: Carbapenems remain the most active agents against Gram-negative isolates, including ESBL producers strain of E. coli and Klebsiella spp. isolated from community-acquired and nosocomial infections from Eastern Romania.
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