14th ICID - Poster Abstracts - International Society for Infectious ...

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When citing these abstracts please use the following reference: Author(s) of abstract. Title of abstract [abstract]. Int J Infect Dis 2010;14S1: Abstract number. Please note that the official publication of the International Journal of Infectious Diseases 2010, Volume 14, Supplement 1 is available electronically on http://www.sciencedirect.com Final Abstract Number: 34.006 Session: Zoonoses and Infectoins in Animals Date: Wednesday, March 10, 2010 Time: 12:30-13:30 Room: Poster & Exhibition Area/Ground Level Type: Poster Presentation Molecular characterization of VP1-3 and NSP1-3 genes of porcine group A rotavirus G12 strain RU172: Evidence for porcine origin of human G12 strains S. Ghosh 1 , N. Kobayashi 1 , M. Ishino 1 , T. Naik 2 1 Sapporo Medical University School of Medicine, Sapporo, Hokkaido, Japan, 2 National Institute of Science Education and Research, Bhubaneshwar, Orissa, India Background: Group A rotavirus is an important cause of infantile diarrhea in humans, and classified into 23 G and 31 P genotypes. Among them, G12 has been regarded as an important emerging genotype of human strains, worldwide. On the other hand, the only G12 strain reported from animals was a G12P[7] strain, RU172, detected in a diarrheic piglet in Eastern India. The VP4, VP6, NSP4 and NSP5 genes of porcine G12 strain RU172 exhibited genetic relatedness to human Wa-like G12 strains. Although the origin of human G12 genotype remains obscure, some recent studies on human G12 rotaviruses suggested that the G12 genotype might be of porcine origin. To determine the true origin of G12 rotaviruses and decipher the exact genetic relatedness between human and porcine G12 strains, we characterized genetically the remaining six genes (VP1-3 and NSP1-3) of porcine G12 strain RU172. Methods: The VP1-3 and NSP1-3 gene sequences of porcine G12 strain RU172 were obtained by RT-PCR and direct sequencing using end primers and several internal primers, designed from conserved stretches of several published sequences. Results: The VP1-3 and NSP1-3 genes of porcine G12 strain RU172 exhibited high sequence identities to Wa-like porcine and human strains, including human G12 strains, and by phylogenetic analyses, clustered within the Wa genogroup along with human Wa-like G12 strains. Conclusion: Wa-like human and porcine group A rotaviruses are believed to be genetically related and have a common origin. Therefore, based on full genome analyses of porcine G12 strain RU172 and human Wa-like G12 strains, we propose that the Wa-like human G12 strains might have resulted from reassortment events involving Wa-like human non-G12 and porcine G12 strains, or more favorably, both the porcine and human Wa-like G12 strains might have evolved from a common progenitor, maybe of porcine origin. The AU-1-like and DS-1-like G12 strains might be the result of reassortment events involving non-G12 strains of these genogroups and human Wa-like G12 strains. Therefore, the present study deciphers the probable origin of human G12 genotype, and provides evidence for porcine-human transmission of rotaviruses.
When citing these abstracts please use the following reference: Author(s) of abstract. Title of abstract [abstract]. Int J Infect Dis 2010;14S1: Abstract number. Please note that the official publication of the International Journal of Infectious Diseases 2010, Volume 14, Supplement 1 is available electronically on http://www.sciencedirect.com Final Abstract Number: 34.007 Session: Zoonoses and Infectoins in Animals Date: Wednesday, March 10, 2010 Time: 12:30-13:30 Room: Poster & Exhibition Area/Ground Level Type: Poster Presentation Veterinary practitioners and the spread of infectious diseases S. T. OLODO-ATITEBI OXFORD BROOKES UNIVERSITY, 0X3 0BP, United Kingdom Background: There is an increasing overlapping among livestock,pets and human beings, highlighting the need for a well defined biosecurity plan to reduce the opportunity for infectious agents to gain access to and spread within a veterinary premises or any other animal housing unit.And the recent outbreaks of infectious diseases around the world have clearly shown the threats to human and animal health arising from emerging and re-emerging infectious disease, a fact which has huge economic and public health implications. Methods: In order to provide the best veterinary care possible, veterinarians have to redefine their underlying responsibility to minimize the risk of additional; harm that might unintentionally befall a patient because of their interventions. Results: This Paper focused on an investigation to dtermine the possible roles of veterinary practitioners in the spread of infectious diseases. Conclusion: This paper focused on an investigation to determine the possible roles of veterinary practitioners in the spread of infectious diseases, discusses the need for biosecurity programs in veterinary practices,and relates a practical approach for developing biosecurity practices that are tailored to individual facilities,to help ensure that veterinary practitioners retain their role in the control rather than the spread of infections.
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