14th ICID - Poster Abstracts - International Society for Infectious ...

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When citing these abstracts please use the following reference: Author(s) of abstract. Title of abstract [abstract]. Int J Infect Dis 2010;14S1: Abstract number. Please note that the official publication of the International Journal of Infectious Diseases 2010, Volume 14, Supplement 1 is available electronically on http://www.sciencedirect.com Final Abstract Number: 34.032 Session: Zoonoses and Infectoins in Animals Date: Wednesday, March 10, 2010 Time: 12:30-13:30 Room: Poster & Exhibition Area/Ground Level Type: Poster Presentation Characterization of Venezuelan field strains of EEEV by RT-PCR and SSCP V. M. Bermudez G., M. C. Gonzalez, J. M. Ruíz , J. Rivero, F. Herrera Central University of Venezuela, College of Veterinary Medicine, AP-4563, Aragua, Venezuela Background: Genetic characterization of three field strains of VEEE, by RT-PCR and SSCP shows difference in virulence, antigenic variation and immune response. Two genetic regions of the virus: )No Trasnducible RNAm 26S and a region of the gen nonstructural protein P4 (nsP4 3 might relate to heterogeneity and behavior of the strains. Methods: EEEV field virulent strains were isolated from epizootic outbreaks in equines and named: Strain 1 or PAO from Cojedes State in 2002. Strain 2 or MERCEDES Guárico State in 1996. Strain 3 or TUCACAS from Falcón State in 1984. Primers from known EEEV were used to copy and to amplify a fragment of 542 pb of the region No Transducible RNAm 26S and 3 another 532 pb fragment of the gen nsP4. Once amplified a RT-PCR was run and DNAc of both fragments from each of the three strains were verified and compared by SSCP. The doble chains of DNAc obtained were denatured and turned into single chains by quick heat and cold stress to promote in chain reassortments or accessory chains. The end products were separated by low amperage PAGE. Results: SSCP of the three strains of EEEV studied showed a wide range of band patterns with marked difference in the migratory pattern of the reassorted singlets (RSS) revealing and showed difference in the migratory patterns of the denatured singlet (DSS). This results uncovered that insertions or deletions of nucleotides took place in the gen sequence of these strains of EEEV. Conclusion: The three field strains of EEEV showed genetic polymorphisms in the genome regions studied which characterize EEEV variants of South America.
When citing these abstracts please use the following reference: Author(s) of abstract. Title of abstract [abstract]. Int J Infect Dis 2010;14S1: Abstract number. Please note that the official publication of the International Journal of Infectious Diseases 2010, Volume 14, Supplement 1 is available electronically on http://www.sciencedirect.com Final Abstract Number: 34.033 Session: Zoonoses and Infectoins in Animals Date: Wednesday, March 10, 2010 Time: 12:30-13:30 Room: Poster & Exhibition Area/Ground Level Type: Poster Presentation Discovery of reverse zoonotic transmission of pandemic H1N1 influenza virus infection in cats following the initiation of a real time sero-molecular epidemiological study J. Trujillo 1 , B. Sponseller 1 , A. Jergens 1 , T. Pearce 1 , E. Strait 1 , K. Harmon 1 , S. Ramamoorthy 1 , C. Prior 2 , A. West 3 , P. Nara 1 1 Iowa State University, Ames, IA, USA, 2 Park City Animal Clinic, Park City, UT, USA, 3 Park City Animal Clinic, Park City, Ut, USA Background: Influenza viruses remain the most significant infectious disease One Health concern entering into 2010. The highly infectious pH1N1 virus is a human host adapted virus with unknown host susceptibly that remains problematic for pandemic influenza preparedness plans. Atypical hosts provide an opportunity for endemic influenza evolution, a new reservoir host, or a site for future mutational events. Following the discovery of the index case for feline pH1N1 infection, we are employing a sero-molecular epidemiological study to monitor infection rates and evolution of this virus at the human-animal contact interface to advance the understanding of influenza virus evolution in populations and predict, prevent or curtail emergent events. Methods: This study utilizes molecular testing; real time RT-PCR differential pH1N1 detection assays; virus isolation, gene sequence analysis for virus detection, typing and viral genomics. Host immune responses are assessed via differential H1N1 ELISA, and specific hemagglutinin inhibition assays and virus neutralization assays. Results: Our epidemiological study discovered the first two feline cases of pH1N1 infections following apparent reverse zoonosis from humans. Both cases include cats older than 10 yrs, clinical signs includ reespiratory disease, lethargy and inappetance, 3-5 days following influenza like illness in humans. One cat was moderately febrile. Both cats developed lower respiratory disease characterized by a bilateral dorsal alveolar pattern on radiography and had lymphopenia. Bronchoalveolar lavage or possibly deep ora-pharyngeal swaps were sufficient for PCR based viral detection. The power of serology in diagnosis will be emphasized. As the study continues, additional cases will be also be presented. Conclusion: Here we report on the discovery and a diagnostic approach of the first pH1N1 virus infection in the atypical domestic cat which was associated with significant lower respiratory disease which is similar to more recent human cases possibly suggesting the discovery of a relevant animal model for future experimental trials with pH1N1 viruses. Moreover these atypical index cases demonstrate the possible development of new endemic infections of influenza virus in close household or community human/animal contacts complicating prepardness plans and reinforcing the need for expanding professionally trained comparative clinical scientists via the One Health initiative.
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