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Mitigation for the Construction and Operation of Libby Dam

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antenna located approximately 5 cm toward <strong>the</strong> posterior <strong>of</strong> <strong>the</strong> incision. The antenna was<br />

threaded through <strong>the</strong> needle, <strong>the</strong> tag inserted into <strong>the</strong> abdominal cavity <strong>and</strong> <strong>the</strong> needle<br />

removed, leaving <strong>the</strong> antenna trailing along <strong>the</strong> burbot’s body. The coded acoustic tags did<br />

not require a hole <strong>for</strong> <strong>the</strong> antenna because <strong>the</strong>y did not have antennae. The incision <strong>for</strong> both<br />

types <strong>of</strong> tags were closed with 4-0 silk using 3-5 stitches. An additional stitch was usually<br />

placed near <strong>the</strong> antenna puncture wound <strong>for</strong> those fish tagged with <strong>the</strong> combined acoustic<br />

<strong>and</strong> radio tags. The entire surgical procedure was usually completed within 3-7 minutes.<br />

After each surgery, <strong>the</strong> fish was allowed to recover in a tank containing fresh water <strong>for</strong> 10-30<br />

minutes <strong>and</strong> <strong>the</strong>n released near <strong>the</strong> site <strong>of</strong> capture.<br />

Tracking ef<strong>for</strong>ts were conducted approximately weekly on <strong>Libby</strong> Reservoir using a<br />

23 foot long Woolridge outboard motorized boat during <strong>the</strong> period <strong>of</strong> mid December 2003 to<br />

May 2004, <strong>and</strong> were limited to daylight hours. We used telemetry receivers manufactured by<br />

Lotek Inc. (Model SRX-400; W7 Firmware) <strong>for</strong> locating both coded acoustic <strong>and</strong> combined<br />

acoustic <strong>and</strong> radio tags. Each receiver unit consisted <strong>of</strong> a radio receiver, data processor,<br />

internal clock, <strong>and</strong> data logger. We used a tuned loop antenna <strong>for</strong> locating <strong>the</strong> radio<br />

component <strong>of</strong> <strong>the</strong> combined acoustic <strong>and</strong> radio tags. For locating <strong>the</strong> coded acoustic tags, we<br />

used an ultrasonic upconverter (model UUCN-150) <strong>and</strong> a hydrophone (model LHP-1)<br />

manufactured by Lotek Inc. that were connected to <strong>the</strong> SRX-400 telemetry receiver. The<br />

ultrasonic upconverter modified <strong>the</strong> 76.8 KHz signal transmitted by <strong>the</strong> acoustic tags <strong>and</strong><br />

converted it 150.077 KHz, which was capable <strong>of</strong> being decoded by <strong>the</strong> telemetry receiver.<br />

We used triangulation methodology to estimate <strong>the</strong> position <strong>of</strong> each tagged fish. We<br />

recorded <strong>the</strong> date, time, approximate depth (m), general location, <strong>and</strong> used a Global<br />

Positioning System (GPS) to identify <strong>and</strong> geo-reference <strong>the</strong> location <strong>of</strong> each tagged fish. We<br />

estimated <strong>the</strong> home range <strong>for</strong> those burbot <strong>for</strong> which we had relocated at least once after<br />

release by measuring <strong>the</strong> fur<strong>the</strong>st distance (along <strong>the</strong> reservoir mid-channel) between<br />

upstream <strong>and</strong> downstream observations.<br />

44

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