Medicinal Plants Classification Biosynthesis and ... - Index of

Biosyntheses and Bioactivities of Flavonoids in the Medicinal...
Absorbance (277 nm)
60
50
40
30
20
10
0
0 50 100 150 200 250 300
Time (min)
Figure 2. HSCCC chromatograms of the crude baicalin extracted from Scutellaria baicalensis Georgi
(A) the first separation, and (B) the second separation [Lu, Jiang & Chen, 2003].
Absorbance (280 nm)
120
100
80
60
40
20
0
0 1 2 3 4 5 6 7 8
Time (h)
baicalin
baicalein
wogonin
oroxylin A
Figure 3. Chromatogram of the crude extract from Scutellaria baicalensis Georgi by HSCCC
separation. Conditions: column, multilayer coil of 2.6 mm i.d. PTFE tube with a total capacity of 325
ml; rotary speed, 1000 rpm; solvent system, n-hexane–ethyl acetate–n-butanol–water (1:1:8:10,
v/v/v/v); mobile phase, lower phase (water phase); flow-rate, 0–4 h, 1.0 ml min -1 and 4–8 h, 2.0 ml min -
1 ; detection at 280 nm; sample size, 500 mg; retention of the stationary phase, 51%. The arrow indicates
the flow-rate of the mobile phase was increased stepwise from 1.0 ml min -1 to 2.0 ml min -1 after 4 h [Li
& Chen, 2005].
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