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Medicinal Plants Classification Biosynthesis and ... - Index of

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Non-Invasive Near Infrared Spectroscopic Techniques for the Characterization...<br />

AU AU<br />

AU<br />

time [min]<br />

b<br />

a<br />

time [min] time [min]<br />

time [min]<br />

Figure 7. Simultaneous determination <strong>of</strong> (a) naphthodianthrones <strong>and</strong> (b) phloroglucines. Conditions:<br />

Hypersil BDS-C18 (5 µm, 130 Å, 250 × 4 mm) ; mobile phase, A: 888.0 g buffer (880.0 g bidest, 2 ml<br />

85% H 3PO 4, TEA (pH 2.80)), 80.0 g ACN, B: 49.64 g buffer (50.0 g bidest, 1 ml 85% H 3PO 4, TEA (pH<br />

6.10)), 85.04 g methanol, 275.28 g ACN; gradient, see Table 2; sample volume, 20 µl; peak assignment,<br />

PrPH, protopseudohypericin; PH, pseudohypericin; CPH, cyclopseudohypericin; PrH, protohypericin;<br />

H, hypericin; Hf, hyperforin; Ahf, adhyperforin.<br />

AU<br />

265

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