Thermo Scientific Orion 2110XP Ammonia Analyzer

CalibrationBefore Performing aDKA CalibrationMaximum system accuracy is ensured through a fast, easy and accuratecalibration performed in the expected sample range. This calibrationprocedure uses equipment supplied in the ammonia calibration kit(Cat. No. 2110XPCAL).Recommendations• Wait at least one hour to perform a calibration after changing thereagent or cleaning the electrode.• Check that the present sample concentration is equal or less thanstandard 1. If not, calibration can still be performed with a loss inaccuracy as long as the background concentration is not significantlylarger than the standard 1 concentration.• Proper pipet technique is crucial to the calibration. If the operator isnot familiar with pipet equipment or technique, refer to the Appendix,Pipet Operation section.• Do not touch the pipet tip with bare fingers. Risk of contamination ispossible. Use powder free gloves to prevent contamination.• When using a pipet to inject standards during calibration, use a freshpipet tip for each standard and each injection.• When a fresh tip is installed, rinse the tip with the same concentrationstandard as the intended injection.• Fill the tip and dispense the standard 1 to 3 times before injecting thestandard into the flow cell. Be sure to discard the standard in thedrain between the rinses.• Wipe any excess liquid from the outside of the tip without touchingthe opening.• Insert the pipet tip into the calibration port on the flow cell cap.Figure IV-4Pipet Dispensing• Be sure to hold the pipet completely vertical while dispensing. Donot inject the standard onto the side of the flow cell, as a film may beretained on the inside of the wall, resulting in calibration precisionerrors. Refer to Figure IV-4.Thermo Scientific Orion 2110XP Ammonia Analyzer User GuideIV-5