OP-II-3

OP-III-A-10•The results showed that increasing residence time (t=V/ V , where V is thecatalyst bed volume andis the volumetric flow rate of the liquid, respectively) from45 s to 90 s doubled R-1-phenylethanol conversion (Fig 2). The conversion wasdecreased after 430 min when the reactant concentration was doubled. After 800 minthe conversion was doubled as expected, due to the decreased volumetric flow rateto the half (Fig 2). No deactivation of the immobilized lipase was observed. Theselectivity of R-1-phenylethyl acetate was 100 %.•VR-1-phenylethyl acetate concentration (M0,0070,0060,0050,0040,0030,0020,0010,000t-= 45 s12- t = 90 s3-t = 45 s40 20 0 400 600 800 10 00Time-on-stream (min)Figure 2. Kinetic resolution of (R/S)-1 at 70 °C. Notation: 1; start-up of the first experiment•using =3 mL min –1 •V, 2; start-up of the second experiment using V =3 mL min –1 till 430 mintime-on-stream, 3; start-up of the third experiment by doubling the reactant concentration••using V=3 mL min –1 till 810 min time-on-stream.Thereafter V =1.5 mL min –1 was applied.The utilization of the continuous flow reactor for the kinetic resolution of racemic1-phenylethanol with ethyl acetate in toluene over an immobilized lipase wassuccessfully demonstrated in this study. In the final presentation transient kinetics,the effect of temperature and different solvents by applying step changes will bedemonstrated. The comparison between the continuous flow reactor and the batchreactor modes for the kinetic resolution of racemic 1-phenylethanol will be discussed.References[1] Bruggink, A; Schoevaart, R; Kieboom, T. Org. Proc. Res. Dev. (2003), 7, 622–640.[2] Jeong, S; Hwang, B-Y; Kim, J; Kim, B-G. J. Mol. Catal. B: Enzymatic (2000), 10, 597-604.[3] Faber, K. Biotransformations In Organic Chemistry, 4th ed., Springer, Berlin, 2004.[4] Poppe, L; Novak, L. Selective Biocatalysis: A Synthetic Approach, VCH: Weinheim-New York, 1992.[5] Bornschauer, U T; Kazlauskas, R J. Hydrolysis In Organic Synthesis: Regio- and StereoselectiveBiotransformation, Wiley-VCH, Weinheim-New York, 2006.170