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PP-<strong>II</strong>I-72HORSERADISH PEROXIDASE IMMOBILIZATION IN CATALYTICOXIDATION OF PHENOLSSulman M., Sidorov A., Lakina N., Sulman E., Tikchonov B.,Matveeva V., Doluda V.Dept. of Biotechnology and Chemistry, Tver Technical University, A. Nikitin str., 22,Tver, 170026 (Russia) sulman@online.tver.ruThe use of the immobilized enzymes in modern industrial catalysis is in greatdemand. These are the major reasons for attaching enzymes to various supports: (i)multiple or repetitive use of a single batch of enzymes; (ii) the ability to stop thereaction rapidly by removing the enzyme from the reaction solution; (iii) enzymes areusually stabilized by binding; (iv) product is not contaminated with the enzyme; (v)analytical purposes - long 1/2-life, predictable decay rates, elimination of reagentpreparation. The surface on which the enzyme is immobilized is responsible forretaining the structure in the enzyme through hydrogen bonding or the formation ofelectron transition complexes. These links will prevent vibration of the enzyme andthus increase thermal stability. The microenvironment of the surface and the enzymehas a charged nature that can cause a shift in the optimum pH of the enzyme of up to2 pH units. Thus, while developing reusable catalytic systems on the basis ofimmobilized enzymes one of the main problems is the choice of the firm carrier forthe immobilization of enzyme which will provide its best stabilization. The applicationof traditional (alumina, silica, ion-exchange resins) and polymer-modified supports isan effective solution to this problem.In this study the catalytic systems on the base of immobilized horseradishperoxidase (HRP) were successfully applied in the reaction of phenol oxidation in thepresence of hydrogen peroxide. Various catalytic systems were synthesized: (i) HRPimmobilized on alumina modified with polyelectrolytes (chitosan (CS) andpoly(sodium 4-styrenesulfonate)), (ii) HRP immobilized on CS-modified cationexchangeand anion-exchange resins. Carbodiimide (CD) and glutaric aldehyde wereused as linking agents.The extraction of HRP was carried out from disintegratedfresh root of horseradish. The activity of the obtained catalyst on the base of ionexchange resins remains practically constant at the repeated application in thereaction of phenol oxidation. Among the catalysts on the base of ion-exchange resinsCS-modified cation-exchanger was the most effective one (99.5 % of phenol574
PP-<strong>II</strong>I-72conversion). Al 2 O 3 /PSS/CS/CD/HRP catalyst revealed 99% of phenol conversion.Thus the catalytic systems obtained can be used for sewage and industrial wastebiocatalytic treatment from dangerous phenolic compounds by their oxidation up toless dangerous biopolymers.AcknowledgementsWe sincerely thank NATO science for peace programme Sfp 981438 for the financialsupport.575
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Boreskov Institute of Catalysisof t
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INTERNATIONAL SCIENTIFIC COMMITTEEV
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SILVER SPONSORThe organizers expres
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PL-1HOW TO DESIGN OPTIMAL CATALYTIC
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MULTIFUNCTIONAL DEVICES FOR INTENSI
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PL-3DESIGN OF CATALYTIC PROCESSES F
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PL-3Indeed preparation and testing
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PL-4The second part of the lecture
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PL-5Reactor designs with intensifie
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PL-6MEMBRANE REACTORS: STATE OF THE
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KEY-NOTE PRESENTATIONS
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KN-1description, that can be charac
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KN-2selective catalytic reduction o
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KN-3Fig. 1. Experimental burner of
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KN-4~200°C. This is based on the H
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KN-5for addressing the quality of t
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KN-6reaction with ethanol and the b
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KN-7at temperature lower than 873K,
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REACTION KINETICS AND REACTION ENHA
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OP-I-2RELATION BETWEEN THE ACTIVATI
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COMPARISON OF CHEMICAL AND ENZYMATI
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OP-I-4NONLINEAR PHENOMENA DURING ME
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OP-I-5SPECIFICITY OF THE OSCILLATIO
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OP-I-6TRENDS IN BISTABILITY DOMAINS
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OP-I-7NON-STEADY-STATE CATALYST CHA
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OP-I-8TRANSIENT KINETIC STUDIES OF
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OP-I-9A REDOX KINETICS FOR THE PART
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OP-I-9500Flow of Air = 0.3 m 3 (STP
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OP-I-11ELUCIDATION OF THE REACTIVIT
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KINETIC MODELLING OF THE JOINT TRAN
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OP-I-13n-HEXANE SKELETAL ISOMERIZAT
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OP-I-14the slow branch of kinetic c
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OP-I-15The hydride palladium comple
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Energy Conservation (field j)∂∂
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OP-I-17Selectivity (mol. %)10099989
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OP-I-18According to GLC the main pr
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OP-I-19and slug dimensions, formati
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OP-I-20A RANS volume of fluids [1]
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OP-I-21the physical-chemical charac
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OP-I-22calculated values of pressur
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OP-I-23where L c - capillary length
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OP-I-24and diffuse transmission thr
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OP-I-25effect of bubble break-up wo
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OP-I-26strong influence on the soli
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OP-I-27wHCOOH=1+K2K1(1 + K3⋅ P⋅
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OP-I-28dimensional profiles detecte
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OP-I-29results. The results of the
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OP-I-30Fig. 1. Hydrodynamics and co
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OP-I-31Convection and diffusion wit
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TEMPERATURE RISE DURING REGENERATIO
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OP-II-2[4]. Similar temperature pro
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OP-II-3(Fig, 1 a, b) upon testing i
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OP-II-4In the second step optimizat
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OP-II-5membrane contactors were stu
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OP-II-6reaction over Cu/CeO 2-x cat
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OP-II-7is modelled by means of a tw
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OP-II-8the reactor [6]. However, th
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OP-II-9Figure 1. Computed volume fr
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OP-II-10assumes variables’ distri
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OP-II-11oxide support, it comes tha
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OP-II-12was connected to G.C. via s
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OP-II-13catalyst bed was 6 mm. Thre
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OP-II-14as coolant is an important
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OP-II-15Flow (L/h)7654321Bottoms ra
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OP-II-16(4) The gas recirculation r
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OP-II-17Catalytic reactions were ca
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OP-II-18At steady surface coverage
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OP-II-19gaimpulsegeneratorwateFig.
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OP-II-20examined (see Figure 1). Th
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OP-II-21References[1]. M.P. Dudukov
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OP-II-22of the hydrogen oxidation a
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OP-II-23TemperaturesensorsThe efflu
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OP-II-24of reactant conversions. Pa
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OP-II-25(surface) ‘wall-reaction
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OP-II-26It was found that the 10 wt
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OP-II-27non-flow type. During the M
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OP-III-A-1A NEW SIMPLE MICROCHANNEL
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BUBBLING FLUIDISED BED PYROLYSIS OF
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PINEWOOD PYROLYSIS UNDER VACUUM CON
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OP-III-A-5PYROLYSIS OF HDPE IN A CO
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OP-III-A-6REACTORS FOR THE GREEN TR
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DEHYDRATION OF GLYCEROL TO ACROLEIN
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OP-III-A-8LACTIC ACID BASED ON BIO
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RECOVERY OF ACETIC ACID FROM PYROLY
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OP-III-A-10LIPASE-CATALYZED REACTIO
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OP-III-A-11INVESTIGATION ON THERMOC
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OP-III-A-12SELECTIVE CATALYTIC DEOX
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ETHANOL STEAM REFORMING OVER COBALT
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OP-III-A-14HYDROTREATMENT CATALYSTS
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ORAL PRESENTATIONSSECTION IIIChemic
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OP-III-B-1Reactor parameters:Intern
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OP-III-B-2JOINT STEAM REFORMING OF
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OP-III-B-3LANDFILL BIOGAS PURIFICAT
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OP-III-B-4MODELING AND SIMULATION O
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OP-III-B-5DemonstratorThe medium-te
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OP-III-B-630/19.6 Nl/min (space vel
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OP-III-B-7mixed in the ratios: 94,8
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OP-III-B-8Table 1.Material balance
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OP-III-B-9Based on the results of t
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OP-III-B-10On this basis it can be
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OP-III-B-11Similar trends caused by
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OP-III-B-12(mol/s g cat)x108r4,6-DM
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OP-III-B-13carbon in Wyoming coal i
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OP-III-B-14MODELING PRODUCT DISTRIB
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OP-III-B-15COMBINED TECHNOLOGY OF U
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MATEMATICAL MODEL FOR DOWNDRAFT BIO
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OP-III-B-17CATALYTIC DEHYDRATION OF
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OP-III-B-18SYNGAS AND HYDROGEN PROD
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POSTER PRESENTATIONSSECTION I
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PP-I-1influence of the direction of
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PP-I-2At the agitation of the liqui
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PP-I-3NOLINEAR PHENOMENA IN CATALYT
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PP-I-4A NEW APPROACH TO KINETIC STU
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PP-I-5KINETICS OF PROX REACTION OVE
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PP-I-6PHENOMENA OF SUPERADIABATIC T
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ELECTROMAGNETIC REACTOR OF WATER TR
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PP-I-8DIRECT SYNTHESIS OF HYDROGEN
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PP-I-9DYNAMICS OF FIRST-ORDER PHASE
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PP-I-10ELECTROCHEMICAL OXIDATION OF
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PP-I-11CHEMPAK SOFTWARE PACKAGE: OP
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PP-I-12COMPUTER SIMULATION OF ENDOT
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PP-I-13MODELLING KINETICS OF PROCES
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PP-I-14THE INVESTIGATION OF REACTIO
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PP-I-15The qualitative picture of s
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Thus, knowing the composition of ra
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PP-I-17current density. Preliminary
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PP-I-18Hydrogenation kinetics was d
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PP-I-19the active mixing, amplitude
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PP-I-20where r, h indicate the radi
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PP-I-21model is very anisotropic be
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PP-I-22- Reduction of the number of
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PP-I-23oscillations of intermediate
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PP-I-25SYNTHESIS OF ETHYLENE OXIDE
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PP-I-26OPTIMUM KINETICS FOR POLYSTY
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PP-I-27TableSpecific surface area (
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PP-I-28γ-preirradiated sample), th
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PP-I-29commercial CFD solver FLUENT
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PP-I-30modulus, a considerable decr
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PP-I-31The destruction ways of CF 3
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PP-I-32[7]. Karoor S, Sirkar K. Gas
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PP-I-33above 750°. The catalytic p
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PP-I-35REVERSE FLOW REACTOR WITH FO
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FLOW-RECIRCULATION METHOD FOR INVES
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TO A PROBLEM OF OPTIMIZATION OF FUN
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PP-I-38THE INFLUENCE OF REACTION MI
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PP-I-39METHANOL OXIDATIVE STEAM REF
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PP-I-41CORRECT INVESTIGATION OF THE
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PP-I-42NEW APPROACH OF DEFINITION O
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PP-I-43STREAM HEAT EXCHANGE OF AERO
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PP-I-44HIGH TEMPERATURE OXYGEN TRAN
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PP-I-45KINETICS OF TRANSESTERIFICAT
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MATHEMATICAL MODELING AND OPTIMIZAT
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PP-I-47The basic side reaction is r
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PP-I-49EXPERIMENTAL STUDY OF THE HA
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PP-I-51ON THE KINETICS AND REGULARI
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PP-I-52DIFFERENTIAL THERMAL ANALYSI
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PP-I-53oxidation reaction demonstra
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PP-I-54of reaction. It is establish
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PP-I-55effects, are energetically c
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PP-I-56The experiments and simulati
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PP-I-57In the studied range of temp
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PP-I-58HeadingsAdvances in Chemical
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PP-I-59calculated. During the aroma
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PP-I-60with honeycomb catalyst) act
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Т - temperature, K, D eff - effect
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PP-I-62stirring rate were adopted u
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PP-I-63m 2 = (k 1 y 0 1 +k -1 +k 1
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POSTER PRESENTATIONSSECTION II
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PP-II-1values of operating paramete
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⎡⎛⎢⎜bF⎣⎝=2ab ⎞ 6+ ⎟
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PP-II-4HONEYCOMB MONOLITHIC CATALYS
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MATHEMATICAL MODELING OF THE ALUMIN
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PP-II-6MODELING OF VINYL ACETATE SY
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PP-II-7СENTRIFUGAL DISK REACTORAvv
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PP-II-83) An opportunity of operati
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PP-II-10UV-ACTIVATION OF METHANE CO
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PP-II-11COMBINED APPROACH (FMEA- HA
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PP-II-12Testing the pilot variant o
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PP-II-14NOVEL MICROREACTOR FOR THE
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PP-II-15of 1-2.5 lead to a decreasi
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PP-II-16stages. Kinetic parameters
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PP-II-17whiskers, which assure an a
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PP-II-18с m , с cat - density of
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PP-II-19decrease. So, the potential
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PP-II-20products became a mixture o
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PP-II-21A numerical algorithm and s
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PP-II-22motions at any external con
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PP-II-23One of the main technologic
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PP-II-24100 m x 250 μm x 0.5 μm,
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PP-II-25A model was developed to ca
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PP-II-26The results of calculations
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PP-II-27modes of reaction and two d
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PP-II-29OZONE-DESTRUCTION REACTOR B
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PP-II-30Fig. 1. Axial profile of me
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PP-II-31isobutylene in butene fract
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PP-II-322.5x10 -3 ( i )2.5x10 -3 (
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PP-II-33No olefinreadsorptionWith o
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PP-II-34using titania or Ag-doped t
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PP-II-35accidents, is condition dep
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PP-II-36be seen that at temperature
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PP-II-37the solid phase solute conc
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PP-II-38As shown in Table 1, the ca
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PP-II-39better to the obtained in t
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PP-II-40Hydrodynamic regime in the
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PP-II-41activity, relative unit1,21
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PP-II-42One of the major indicators
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PP-II-43These conditions are confor
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PP-II-44For the hepten, alpha-methy
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PP-II-46PARTIAL OXIDATION OF METHAN
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PP-II-47DEVELOPMENT OF VORTEX APPAR
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MODELING OF CFTALYTIC α-OLEFINS OL
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PP-II-49Hinselwood kinetic equation
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3) iterate concentrations at a cut
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PP-II-51data such flow sheet provid
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PP-II-53ON THE TECNOLOGY OF TECHNIC
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PP-II-54PROPYLENE POLYMERIZATION AN
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REACTOR FOR LIQUID-PHASE PROCESSES
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PP-II-56MODELING OF CATALYTIC MICRO
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PP-II-57MATHEMATICAL MODELING OF BE
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PP-II-58HONEYCOMB CATALYSTS WITH PO
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POSTER PRESENTATIONSSECTION IIISECT
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ReferencesPP-III-1[1]. A.N. Pestrya
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PP-III-2Table1. Percentages of diff
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PP-III-3conversion for the reaction
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PP-III-4900Reformer temperature (K)
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PP-III-5Catalytic performance of th
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PP-III-6optimization permit better
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PP-III-8DE-NO X SYSTEM BASED ON H 2
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PP-III-9SEPARATION BETWEEN CHLORIDE
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CONVERSION OF WASTE COTTON TO BIOET
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PP-III-12THE METHOD OF GLYCERIC ACI
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NOx conversion vs. temperature is p
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PP-III-14intermetallic diffusion at
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PP-III-15The conversion of O 2 was
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PP-III-16H 2consumpition (a.u)(a)39
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PP-III-17the characteristic structu
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PP-III-18Figure 1. Influence of tem
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PP-III-19(a)(b)CH 4conversion, %100
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PP-III-20take into account own size
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PP-III-2110080CS-18CS-34CHZ30CHZ801
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EXPERIMENTALPP-III-22We synthesized
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PP-III-23Base composition component
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PP-III-25CO REMOVAL AT THE MICROSCA
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HYDROGEN PRODUCTION FROM METHANOL U
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PP-III-27NON CATALITIC PRODUCTION O
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PP-III-28Different reaction conditi
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PP-III-29followed by WGS reaction.
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PP-III-30As a solvent-coreactants w
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PP-III-31It was concluded that on t
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PP-III-32Hydrogenolysis of glycerol
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PP-III-331,6W 0, μmol Н 2/min1,20
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PP-III-34preparation (glass fiber f
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PP-III-35In the modification proces
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PP-III-36The kinetics of acid hydro
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PP-III-37reaction: i) C=O double bo
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PP-III-38methane and carbon monoxid
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PP-III-39from 25 to 60, which was d
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PP-III-401% w/w for Pd with respect
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PP-III-41material. Besides, the mol
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PP-III-43METHANOL AND DIMETHYL ETHE
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PP-III-44goal is attained by using
Page 523 and 524: OLIGOMERISATION OF TERTIARY AMINE L
Page 525 and 526: PP-III-47meter (Shimazu Co.; TOC-50
Page 527 and 528: PP-III-48the range of 29-87 kJ/mol
Page 529 and 530: PP-III-49determine the optimal type
Page 531 and 532: PP-III-50NEW CONCEPT FOR A SELF CLE
Page 533 and 534: PP-III-51HYDROGEN PRODUCTION BY MET
Page 535 and 536: PP-III-52CATALYTIC UPGRADING OF PRO
Page 537 and 538: PP-III-53CATALYTIC CONVERSION OF FI
Page 539 and 540: PROCESS FOR THE PRODUCTION OF BUTYL
Page 541 and 542: PP-III-55been previously fixed and
Page 543 and 544: PP-III-56The influence of reaction
Page 545 and 546: PP-III-57sample does not contain so
Page 547 and 548: PP-III-58The re-activation of the e
Page 549 and 550: PP-III-59The aim of this work is th
Page 551 and 552: PP-III-61BIODIESEL FROM MICROALGAE
Page 553 and 554: DEVELOPING MICROFLUIDIC DEVICE WITH
Page 555 and 556: THREE-PHASE DIRECT OILS HYDROGENATI
Page 557 and 558: Co-BASED CATALYSTS FOR THE HYDROLYS
Page 559 and 560: PP-III-65KINETIC STUDY OF THE CATAL
Page 561 and 562: PP-III-66PRODUCTION OF HYDROGEN AND
Page 563 and 564: PP-III-67ENHANCED GASIFICATION OF P
Page 565 and 566: PP-III-68INFLUENCE OF SPILLOVER ON
Page 567 and 568: PP-III-69AEROBIC OXIDATIVE COUPLING
Page 569 and 570: PP-III-70MECHANISMS FOR CHEMICAL RE
Page 571 and 572: PP-III-70SBS molecules and increase
Page 573: PP-III-71and as a resut, could enha
Page 577 and 578: PP-III-73SBA50TiSBA% Transmittance5
Page 579 and 580: PP-III-74stove heating. Another par
Page 581 and 582: PP-III-75TG [%]0-10-20-30380°C400
Page 583 and 584: PP-III-76The elementary version of
Page 585 and 586: PP-III-77thermodynamically enhanced
Page 587 and 588: PP-III-78GFC textileStructuringmeta
Page 589 and 590: PP-III-79All the prepared spinel-ox
Page 591 and 592: PP-III-80Our new process is polluti
Page 593 and 594: PP-III-81Fig. 1. Scheme of the biog
Page 595 and 596: PP-III-82Pic. 1 Pic. 2Pic. 1. Schem
Page 597 and 598: ARKHIPOV Vladimir AfanasievichResea
Page 599 and 600: CENTENO Felipe OrlandoNúcleo de Ex
Page 601 and 602: FLID Mark RafailovichScientific Res
Page 603 and 604: HOSEN Mohammad AnwarUniversity of M
Page 605 and 606: KOZLOVA EkaterinaBoreskov Institute
Page 607 and 608: MAKARSHIN Lev LvovichBoreskov Insti
Page 609 and 610: ONSAN Zeynep IlsenDepartment of Che
Page 611 and 612: REBOLLAR MoisesInstituto De Investi
Page 613 and 614: SHEIKH Munir AhmedTraining and Staf
Page 615 and 616: SULMAN Esfir MikhailovnaTver Techni
Page 617 and 618: ZHAPBASBYEV Uzak KairbekovichKazakh
Page 619 and 620: OP-I-3 Pécar D., Gorsek A.COMPARIS
Page 621 and 622: OP-II-3Kucherov A.V., Finashina E.D
Page 623 and 624: OP-III-A-9 Rasrendra C.B., Girisuta
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PP-I-9Bykov V., Tsybenova S.B.DYNAM
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PP-I-43 Pechenegov Y.Y., Kuzmina R.
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PP-II-10 Basov N.L., Oreshkin I., T
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PP-II-45 Stepanek J., Koci P., Kubi
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PP-III-19 Fedorova Z.A., Danilova M
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PP-III-53 Pölczmann G., Valyon J.,
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XIX International Conference on Che
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