OP-II-3

OP-I-3example the FTIR spectra collected during one selected experiment is presented inFig. 1. It can be clearly seen that the intensity of the broad peak (absorbance, A)within the wavenumber range, ν = (978–1096) cm –1 increases over the reaction time.After a certain period of time, depending on experimental conditions, stady state isformed.Figure 1: Waterfall plots of FTIR spectra during the sucrose hydrolysis at reactionmixture temperature, ϑ = 50 °C and volume flow rate, q V = 7 mL⋅min –1(shown with different angel of sight).The FTIR spectra were transformed to the real-time molar concentration profilesof sucrose considering peak areas treatment of the corresponding peak with the twopoint base line correction. The concentration of sucrose, glucose and fructose in thereaction mixture was determined using calibration curve.Data obtained from the experiments yielded, through application of mathematicalmodeling, kinetic information such as rate constant for chemical reaction, Michaelis-Menten parameters for enzymatic reaction, activation energy and the reaction ratesfor both reactions. All values were compared to previously published ones.Determining reaction kinetics is one of the most crucial development step in reactorsystem design. In order to achieve the highest accuracy and validity regarding kineticmeasurements in the chemical, biochemical, food and pharmaceutical industrieshigh-quality and up-to date equipment is necessary. With presented analysis it wasverified that our bench-top packed bed reactor is adequate to perform kinetic studies,particularly if it is used in combination with FTIR, one of the most powerful, provenand widely used PAT tool. Also the advantages of enzymatic reaction were exposed.References[1]. L.D.S. Marquez, B.V. Cabral, F.F. Freitas, V.L. Cardoso, E.J. Ribeiro, Journal of MolecularCatalysis B: Enzymatic, 2008 51 86-92.[2]. S. Kumar, V. S. Chauhan, P. Nahar, Enzyme and Microbial Technology, 2008 43 517-522.[3]. A. C. S. de Almeida et. al., Electronic Journal of Biotechnology, 2005, 8, 55-62.42