OP-II-3

PP-I-62STUDY ON EXOENZYMATIC KINETICS OF AUTOTHERMALTHERMOPHILIC AEROBIC DIGESTION FOR PRE-TREATINGKLEBSIELLA PNEUMONIAEJing-Ming Liu a,b , Dong-Dong Sun a , Hui Liu a , Ying-Bing Nie c , Zhirong Zhu b, *a School of Chemical Engineering, Northeast Dianli University, Jilin 132012, China.b Department of Chemistry, Tongji University, Shanghai 200092, China.c Jilin Vocational College of Industry and Technology, Jilin132013,China.*Corresponding author: zhuzhirong@tongji.edu.cnIntroduction1, 3-propanediol (1, 3-PD) is an important chemical, and the bio-fermentation forproducing 1, 3-PD by Klebsiella Pneumoniae is also exclusively the most efficientprocess [1] . Klebsiella Pneumoniae strains belong to genus Klebsiella, which is a kind ofgram stain-negative and opportunistic pathogen and can cause a large number ofserious diseases. The traditional way to treat infectious pollutants is to incinerate them,wasting a lot of energy and polluting environment. Autothermal Thermophilic AerobicDigestion (ATAD) has a self-heating ability and removes pathogens with exoenzymesof theromphiles at a temperature of 55° [2] . In order to provide an innovative guidanceof wastewater treatment and engineering design, a laboratory-scale test of the ATADfor pre-treating abandoned strains of Klebsiella Pneumoniae is investigated and a newdynamic model is established with a showing temperature change.ExperimentalAfter the waste strains were concentrated by using a gravity thickening method,enzymatic kinetics of the ATAD was carried out at the facultative oxygen, in abatched-designed biochemical reactor with a main self-heating. In order to establish anexoenzymatic kinetic model of empirical Arrhenius-typed equation and avoid theexisting equation shortcomings without the temperature changes and other factors,four factors related to reaction temperature, the TSS concentrations, aeration rate, andstirring rate were considered. Corresponding coefficients of the equation are obtainedby ATAD test and the least squares regression. The temperature, aeration rate and330