Development of Karl Fischer Reagents
Development of Karl Fischer Reagents
Development of Karl Fischer Reagents
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Chapter III: Applications - 2<br />
[Examples <strong>of</strong> Measurement]<br />
(1) Volumetric titration<br />
<strong>Reagents</strong> used: <strong>Karl</strong> <strong>Fischer</strong> Reagent SS-Z (or SS)<br />
Dehydrated Solvent GEX, SU (or MS, FM) 25-50ml<br />
Substance<br />
Dehydrated<br />
solvents<br />
Sample Quantity<br />
(g)<br />
Measurement Value<br />
(mg)<br />
Moisture Content<br />
(%)<br />
L-alanine General-use 0.5316 0.13 245ppm<br />
Potassium asparaginate " 0.1026 5.29 5.16<br />
Glycylglycine " 0.2882 0.24 833ppm<br />
Vitamin B12 " 0.1452 11.27 7.76<br />
Vitamin B1 hydrochloride " 0.2321 9.89 4.26<br />
ATP Sugar-type 0.0750 6.55 8.73<br />
Citidine (5') diphosphocholine sodium " 0.0466 1.92 4.12<br />
Dextran " 0.0901 5.93 6.58<br />
Chlorella " 0.0550 3.54 6.44<br />
Yeast extract " 0.0810 0.95 3.58<br />
Cyclodextrin " 0.0386 1.55 4.02<br />
(2) Moisture vaporization - Volumetric titration<br />
<strong>Reagents</strong> used: <strong>Karl</strong> <strong>Fischer</strong> Reagent SS-Z (or SS)<br />
Dehydrated Solvent GEX (or MS) + PG (3:1) 50-100ml<br />
Substance<br />
Heating Temperature<br />
( � C)<br />
Sample Quantity<br />
(g)<br />
Measurement Value<br />
(mg)<br />
Moisture Content<br />
(%)<br />
Adenosine triphosphate 200 0.0430 3.28 8.41<br />
(3) Moisture vaporization - Coulometric titration<br />
<strong>Reagents</strong> used: Aquamicron AX (or AS) 150ml<br />
Aquamicron CXU 10ml<br />
Substance<br />
Heating Temperature<br />
( � C)<br />
Sample Quantity<br />
(g)<br />
Measurement Value<br />
(µg)<br />
Moisture Content<br />
(%)<br />
L-alanine 150 0.7408 56 076ppm<br />
Sodium asparaginate 180 0.0366 2020 5.52<br />
Glycylglycine 130 0.2765 70 253ppm<br />
L-cystine 130 0.1123 796 0.709<br />
Dextran 180 0.0457 3093 6.76<br />
Vitamin preparations 110 0.1211 2235 1.85<br />
Vitamin C 100 0.1264 1275 1.01<br />
Vitamin B12 130 0.0403 3128 7.76<br />
Chlorella 150 0.0659 4180 6.34<br />
Yeast extract 80 0.0391 1446 3.70<br />
Yeast nucleic acid 200 0.0230 5875 25.5<br />
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