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Development of Karl Fischer Reagents

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I-1. Questions & Answers about <strong>Karl</strong> <strong>Fischer</strong> <strong>Reagents</strong><br />

3. Notes to Official Food Additive List (7th ed., 1999)<br />

62<br />

Sorbitan sesquioleate 0.5% (1g)<br />

Propylene glycol 0.5% (2g)<br />

Polysorbate 80 3.0% (1g, back titration)<br />

Sodium lauryl sulfate 5.0% (0.5g)<br />

Polyvinyl pyrrolidone K25 5.0% (1.0g)<br />

Polyvinyl pyrrolidone K30 5.0% (1.0g)<br />

Polyvinyl pyrrolidone K90 5.0% (1.0g)<br />

Macrogol 400 1.0% (2g)<br />

Macrogol 1500 1.0% (2g)<br />

Macrogol 4000 1.0% (2g)<br />

Macrogol 6000 1.0% (2g)<br />

Berberine tannate 6.0% (0.7g)<br />

General Test Method 17: Moisture Content Test (<strong>Karl</strong> <strong>Fischer</strong> Method)<br />

The <strong>Karl</strong> <strong>Fischer</strong> method is stipulated for the following food additives.<br />

Item Tolerance<br />

5'-Inosinic acid sodium salt 29.0% or lower (0.15g, back titration)<br />

5'-Uridine acid sodium salt 26.0% or lower (0.15g, back titration)<br />

Citric acid (anhydrous) 0.5% or lower (2g)<br />

Disodium glycyrrhizate 13.0% or lower (0.2g, back titration)<br />

5'-Cytidine acid sodium salt 26.0% or lower (0.15g, back titration)<br />

Sucrose fatty acid ester 4.0% or lower (back titration)<br />

Sodium dehyroacetate 8.3-10.0% or lower (0.3g, back titration)<br />

Folic acid 8.5% or lower (0.2g, back titration)<br />

5'-Calcium ribonucleotide 23.0% or lower (0.15g, back titration)<br />

5'-Sodium ribonucleotide 27.0% or lower (0.15g, back titration)<br />

4. Japan Petroleum Institute Standards<br />

Test for water in liquid petroleum products (<strong>Karl</strong> <strong>Fischer</strong> Method)<br />

JPI-5S-14-76

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