Development of Karl Fischer Reagents

Substance
Dehydrated
solvents
Sample Quantity
(g)
Chapter III: Applications - 2
Measurement Value
(mg)
Moisture Content
(%)
Pills (heart tonic) General-use 0.1356 8.86 6.53
Muscle " 0.1018 75.74 74.4
Brain " 0.0994 77.23 77.7
Blood serum " 0.0916 83.90 91.6
Pancreas " 0.6645 33.36 5.02
Bile acid " 0.9185 15.15 1.65
Licorice powder " 0.1111 9.91 8.92
Crude drugs Sugar-type 0.0608 18.80 30.9
Capsules " (40 � C) 0.0697 9.81 14.1
Soft capsules " (40� C) 0.0528 5.09 9.64
(2) Moisture vaporization - Volumetric titration
Reagents used: Karl Fischer Reagent SS-Z (or SS)
Dehydrated Solvent GEX (or MS) + PG (3:1) 50-100ml
Substance
Pills (heart tonic)
Crude drugs
Medicinal wafers
Capsules
Toothpaste
Black hair
Blond hair
Heating Temperature
( � C)
110
130
150
170
200
200
200
Sample Quantity
(g)
0.2215
0.0532
0.0776
0.0726
0.2069
0.0726
0.1583
(3) Moisture vaporization - Coulometric titration
Reagents used: Aquamicron AX (or AS) 150ml
Aquamicron CXU 10ml
Substance
Heating Temperature
( � C)
Sample Quantity
(g)
Measurement Value
(mg)
14.51
16.46
12.82
10.14
64.33
08.09
18.38
Measurement Value
(µg)
Moisture Content
(%)
06.55
30.90
16.50
14.00
31.10
11.10
11.60
Moisture Content
(%)
Crude drugs 130 0.0961 1254 1.30
Licorice powder 130 0.0439 3977 9.06
Cinnamon 130 0.0620 3464 5.59
Mint 110 0.0428 2411 5.63
Soft capsules 150 0.0463 4463 9.64
Gelatine capsules 150 0.0201 3262 16.2
185