8th Liquid Matter Conference September 6-10, 2011 Wien, Austria ...

P6.42Fri 911:10-14:00Interactions of alkylphosphocholines (ACP) withmembrane lipids - the Langmuir monolayer studyAnita Wnetrzak, 1 Kazimierz Latka, 1 and Patrycja Dynarowicz - Latka 11 Jagiellonian University, Institute of Physics, Reymonta 4, 30-059, Cracow, PolandAlkylphosphocholines (APCs) belong to a class of synthetic antitumor lipids (ATLs), whichare new generation anticancer drugs [1]. In contrast to traditional antitumor substances, theydo not attack the cell nucleus, but the cell membrane. Therefore, it is interesting to studyhow APCs interact with cellular membrane components. Three alkylphosphocholines, namelyhexadecylphosphocholine (miltefosine), octadecylphosphocholine and erucylphosphocholine,were chosen for investigations. Their interactions with main membrane lipids (cholesterol, DPPC,POPC and sphingomyelin) were studied with the Langmuir monolayer technique [2], whichserves as a biomembrane model [3]. The surface pressure (pi) and vs mean molecular area (A)isotherms were recorded for the investigated APC/membrane lipid mixture. The interactions wereanalyzed both qualitatively (with mean molecular area (A12)-, excess area of mixing (Aexc)-composition plots) and quantitatively (with dGexc function).[1] A. H. van der Luit, S. R. Vink, J. Klareenbeek, D. Perrissoud, E. Solary, M. Verheij,W. J. van Blitterswijk, Mol Cancer Ther 6(8), 2337-45 (2007).[2] G. L. Gaines, Jr, Interscience, (1966).[3] R. Maget-Dana, Biochim. Biophys. Acta, 1462, 109-140 (1999).42