8th Liquid Matter Conference September 6-10, 2011 Wien, Austria ...

Thu 811:10-14:00P7.43Adsorption of proteins on polyelectrolyte brushes andmetal surfacesLudger Harnau, 1 Katja Henzler, 1 Matthias Ballauff, 2 Stephan Rauschenbach, 2and Klaus Kern 31 Universitaet Stuttgart, Institut fuer Theoretische und Angewandte Physik, Pfaffenwaldring57 D-70569, Stuttgart, Germany2 Helmholtz-Zentrum Berlin fuer Materialien und Energie GmbH, Berlin, Germany3 Max-Planck-Institut for Solid State Research, Stuttgart, GermanyThe adsorption and immobilization of proteins on solid surfaces is among the most importantproblems in biochemical research given that many biologically processes occur at interfaces.Various biotechnological processes require immobilization of enzymes with full retention oftheir biological activity. In combined experimental and theoretical efforts the adsorption ofproteins on spherical polyelectrolyte brushes [1, 2, 3] and on metal surfaces [4, 5] is studied. Thetheoretical modeling of time-resolved small-angle x-ray scattering revealed that the uptake of theproteins into a spherical polyelectrolyte brush shell is relatively fast and the final stage is reachedwithin three seconds. A similar directed motion of proteins and other biomolecules throughbiological membranes could play a pivotal role in their self-assembly. On metal surfaces two- andthree-dimensionally folded proteins as well as unfolded proteins can be found depending on theinteraction between the proteins and the substrate. Electrospray ion beam deposition allows for aconformational selective adsorption of proteins. This opens the possibility for functional proteinstructures at surfaces.[1] Directed motion of proteins along tethered polyelectrolytes, Phys. Rev. Lett. 100.158301 (2008).[2] Adsorption of beta-lactoglobulin on spherical polyelectrolyte brushes, J. Am. Chem. Soc.132, 3159 (2010).[3] Interaction strength between proteins and polyelectrolyte brushes, Phys. Chem. Chem. Phys.(2011).[4] Electrospray ion beam deposition of clusters and biomolecules, Small 2, 540 (2006).[5] Two- and three-dimensionally folded proteins on metal surfaces in vacuum, Preprint (2011).43