8th Liquid Matter Conference September 6-10, 2011 Wien, Austria ...

Tue 611:23-14:00P10.5One-step methodology for integration of actives innanovesicles using compressed fluidsIngrid Cabrera, 1 Elisa Elizondo, 1 Olga Esteban, 2 Jose Luis Corchero, 3 MartaMelgarejo, 4 Daniel Pulido, 4 Alba Córdoba, 4 Evelyn Moreno, 1 Esther Vazquez, 3Fernando Albericio, 4 Miriam Royo, 4 Antonio Villlaverde, 3 Maria Parajo, 2 NoraVentosa, 4 and Jaume Veciana 11 ICMAB-CSIC, Campus Universitari de Bellaterra, 08193, Barcelona, Spain2 IBEC, Barcelona, Spain3 IBB, Barcelona, Spain4 Institut de Recerca Biomédica, Barcelona, SpainSmall unilamellar vesicles (SUVs) have gained a lot of attention in the drug delivery field becausethey can be used as smart nanocapsules with a precise response to external stimuli and can befunctionalized to obtain active drug delivery systems [1]. Methods to produce SUVs includecommonly used techniques (thin film hydration, etc) and a further post-formation step (sonication,etc). These processes are generally complex, time consuming, multistep and not easily scalable.Furthermore toxic solvent residue can remain in the final product, and bioactivity lost of thedrug might occur. Compressed fluids (CFs)-based methodologies emerged in the 90s as analternative to the use of conventional liquid solvent. The special characteristics of CFs betweenthose of liquids and gases permit the straightforward production of materials with high structuralhomogeneity, which is very important for optimum performance of functional materials. In thatcontext we have been using CO 2 -expanded solvents for the preparation of molecular materialswith controlled supra, nano and micro structure [2]. Here we present a simple and easy to scaleup one-step methodology to integrate biomolecules of different nature (peptides, proteins, etc) insmall unilamellar vesicles using compressed CO 2 . The resulting nanoconjugates were very stable,homogeneous and structurally well defined. This new methodology allows partial reduction oforganic solvents consumption and there is no lost of activity in biomolecules integrated. Theprocess can provide sterile operating conditions that can be easily transferred to large-scaleoperation with a high batch-to-batch consistency.[1] Sawant, R. , Torchilin, V. , Liposomes as smart pharmaceutical nanocarriers, Soft Matter,(2010) 6, 4026-4044[2] Cano-Sarabia, M. , Ventosa, N. , Sala, S. , Patiño, C. , Arranz, Rocio, Veciana, J. , Preparationof uniform rich cholesterol unilamellar nanovesicles using CO 2 -expanded solvents, Langmuir(2008) 24, 2433-24375