Histopathology of Seed-Borne Infections - Applied Research Center ...

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Microtechniques in Seed Histopathology 267Dissolve gelatin in water at 30°C. When completely dissolved, add phenolcrystals and glycerin.Meyer’s AdhesiveWhite of fresh egg 50 mlGlycerin 50 mlSodium salicylate 1 gShake the mixture and filter through sterile cotton or cheese cloth.Gloy SolutionDistilled water 10 mlGloy (liquid) 2 dropsPotassium dichromate small crystal or a pinchShake the mixture vigorously. Do not store for long.10.5.3 MOUNTING MEDIA10.5.3.1 Aqueous Mounting MediaLactophenolLactic acidGlycerinPhenol crystals or distilled waterMix the above reagents in a ratio of 1:1:1:1 or 2:1:1:1.The use of phenol has been found to be hazardous in laboratories in Swedenand Denmark, and use should be avoided. The mixture of lactic acid, glycerol, andwater (1:2:1) has been found suitable (Mathur and Kongsdal, 2003). A safe and goodmedium for temporary mounts is 2% aqueous glycerin.Lactophenol Cotton Blue0.1 g aniline blue (cotton blue) in 100 ml lactophenolStaining, clearing, and mounting are achieved in one step.Polyvinyl Alcohol (PVA)Polyvinyl alcohol 1.66 gDistilled water 10 mlLactic acid 10 mlGlycerol 1 mlAdd polyvinyl alcohol slowly to the water. Stir on magnetic stirrer. Dissolve for1 to 2 hours. Add lactic acid, stirring vigorously, followed by glycerol.
268 Histopathology of Seed-Borne InfectionsGlycerin JellyGelatin (pure and high quality) 1 part by weightDistilled water 6 parts by weightGlycerin 7 parts by weightPhenol crystals 1 g per 100 g mixtureDissolve gelatin in distilled water for 2 hours or longer. Add glycerin and phenolcrystals. Warm for 15 minutes, stirring continuously. Filter through cheese cloth ina wide-mouth bottle. The mixture solidifies on cooling. Take a small piece of jellyonto a slide, gently warm, mount the material, and place the coverglass.Note: Slides mounted in aqueous mounting media or other temporary liquidmedia may be sealed with nail polish.10.5.3.2 Nonaqueous Mounting MediaCanada balsamCaedaxDPXThe authors prefer to use dilute caedax solution: 2 parts of caedax and 1 partof xylene.10.5.4 STAINSSafranin OAlcoholic solution95% ethyl alcohol 100 mlSafranin 1 gDilute with equal amount of distilled water when the solution needs to be used.Methyl cellosolve solutionSafranin 2 gMethyl cellosolve 100 mlEthyl alcohol 95% 50 mlDistilled water 50 mlSodium acetate 2 gFormalin 4 mlDissolve safranin in methyl cellosolve. Add alcohol, distilled water, and otherchemicals. Stir and store.This solution produces a sharp contrast in stained tissues.
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Histopathology ofSeed-Borne Infecti
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PrefaceThe book deals with only one
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The AuthorsDalbir Singh, Ph.D., for
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ContentsChapter 1 Introduction ....
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4.3.2 Routes for Infection from Ova
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8.4 Survival in Seed ..............
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2 Histopathology of Seed-Borne Infe
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4 Histopathology of Seed-Borne Infe
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2Reproductive Structuresand Seed Fo
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Reproductive Structures and Seed Fo
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14 Histopathology of Seed-Borne Inf
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3Structure of SeedsThe seed habit i
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Structure of Seeds 49(1971) of usin
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Structure of Seeds 51is found in as
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Structure of Seeds 53dry seed of Ly
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Structure of Seeds 553.3.2 SEED COA
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Structure of Seeds 57Endosperm: One
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Structure of Seeds 59scendembepsmuA
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Structure of Seeds 61Endosperm: Sca
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Structure of Seeds 63Chalaza simple
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Structure of Seeds 65stystypmerA Bc
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Structure of Seeds 67epsent endCADB
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Structure of Seeds 69perhscendembpe
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Structure of Seeds 71eposgepi′epo
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Structure of Seeds 73(Zea and Sorgh
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Structure of Seeds 75Baker, D.M. an
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Structure of Seeds 77Maheshwari Dev
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Structure of Seeds 79Vries, M.A. de
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100 Histopathology of Seed-Borne In
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TABLE 5.6Location of Mycelium of St
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7Seed Infection by VirusesViruses a
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Seed Infection by Viruses 201TABLE
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Seed Infection by Viruses 205Unlike
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Seed Infection by Viruses 2073. The
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Seed Infection by Viruses 209Wilcox
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Seed Infection by Viruses 213floret
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Seed Infection by Viruses 215ptowvv
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