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Histopathology of Seed-Borne Infections - Applied Research Center ...

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Index 277transmission electron microscopy, 3, 13, 241Microsporangium and microgenesis, 11–13Microtechniquesadhesives, 266–267aqueous mounting media, 267–268basics, 261component plating, 262dehydration, 264, 265embedding, 265fixatives, 264, 266freehand sections, 264infiltration, 265internal mycelium identification, 262material choice, 261microtomy, 264–266mounting, 265–268nonaqueous mounting media, 268reagent preparation, 266–269sectioning, 265–266seed s<strong>of</strong>tening, 262–263s<strong>of</strong>tening, 265staining, 266, 268–269storage, 264whole-mount method, 263–264Microtomy, 264–266Millet, 7Mimosoideae, 54Mineral deficiency, 249–251Mixed infections, 105, 107, 107Momordica, 61Mothbean, 104Mounting, 265–268Movement, viral infection, 206–209Mustardbacterial infection, 192colonization, 132, 141host–pathogen interactions, 104humidity effects, 251severity <strong>of</strong> infection, 102Mycelium viability, 151Mycospaerella, 94Myristicaceae, 31NNajas lacerata, 27Narcissus, 11Navy beans, 221Necrosis, 255, 256Necrotrophs, 82, 155Nectaries, 17, 90Nematode infectionAnguina agropyronifloris, 238Anguina agrostis, 235–238Anguina tritici, 231–235Aphelenchoides arachidis, 239–241Aphelenchoides besseyi, 239bacteria association, 241–242, 243bacterial infection, 19, 173, 188basics, 3, 229, 244bent grass gall nematode, 235–238Ditylenchus destructor, 238–239, 240ear cockle disease, 231–235histopathology, 231–241penetration, 229–230potato nematodes, 238–239, 240Pratylenchus brachyurus, 241survival in seed, 242testa nematode, 239–241western wheatgrass nematode, 238white tip nematode, 239Nicotiana spp., 17, 66Nonaqueous mounting media, 268Nonpathogenic infections, see Physiogenic andnonpathogenic disordersNonstromatic infection, 151, 152–153Nucellar tracheids, 20Nucellus, 23, 28–29, 73Nuclear endosperm, 25, see also EndospermNymphaea, 53OOats, 123, 231, see also Avena spp.Oilseed, 145Olacaceae, 20, 47Old Dominion, 49Onion, 9, 134, 244, see also AlliumOomycetes, 2, 107–112Orchidaceae, 2, 27, 53Orchidaceae, 20Orobanchaceae, 27, 54Oryza spp.flowers, 9ovules, 20seed coat, 40, 49seeds, 47seed structure, 70, 73Osmophors, 11Ovariesbacterial infection, 174, 175, 176basics, 14, 15content replacement, 7fungi, 83–91, 93–94, 95Ovary gall, 235, 237Ovulesbacterial infection, 169basics, 17, 18

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