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S02<br />

for mutagenic induction on the mandarins ‘Anana’, ‘Vietnam’ and ‘Kara’. Seeds, budwood and seedlings were<br />

irradiated with a 6o Co gamma source with five doses (25Gy, 30Gy, 50Gy, 70Gy, 90Gy). No significant difference<br />

in radio sensitivity was observed between varieties depending on the dose of treatment. Single dose 90Gy<br />

caused the decrease of buds viability. Peeled seed showed no differences in radio sensitivity. Some stimulation<br />

of germination rate and early growth was observed with low doses. The LD 50 for seedlings was between 70Gy<br />

and 90Gy. Field evaluation of the obtained mutants is currently performed in Kabbage domain.<br />

S02P30<br />

Effect of budwood irradiation on seed number of clementine, ‘Nova’ and ‘Robinson’ mandarins<br />

Kafa G., Seday U., Uysal O., and Polatöz S.<br />

Alata Horticultural Research Station Mersin (AHRS), Turkey. gucerkafa@gmail.com<br />

We have investigated the effect of budwood irradiation on seed number of clementine, ‘Nova’ and ‘Robinson’<br />

mandarin derived mutant populations. A total 675 plants issued from budwood irradiation and 10 nonirradiated<br />

plants of each parental variety as controls were planted in the field. For two years, seed number of<br />

135 clementine mutants, 110 ‘Nova’ mutants, 165 ‘Robinson’ mutants and control plants were determined.<br />

Clementine mutant plants average seed number was 15.22 for 30 gray, 18.43 for 50 gray and 18.28 for nonirradiated<br />

in 2010. ‘Nova’ mutant plants average seed number was 8.25 for 30 gray, 7.36 for 50 gray and 18.38<br />

for non-irradiated in 2010. ‘Robinson’ mutant plants average seed number was 10.75 for 30 gray, 11.76 for 50<br />

gray and 23.66 for non-irradiated in 2010. Clementine mutant plants average seed number was 17.10 for 30<br />

gray, 17.19 for 50 gray and 17.54 for non-irradiated in 2011. ‘Nova’ mutant plants average seed number was<br />

11.01 for 30 gray, 15.53 for 50 gray and 18.61 for non-irradiated in 2011. ‘Robinson’ mutant plants average<br />

seed number was 11.32 for 30 gray, 12.11 for 50 gray and 22.38 for non-irradiated in 2011. As a result budwood<br />

irradiation decreases seed number in resulting mutant plants, especially for ‘Nova’ and ‘Robinson’ mandarins.<br />

S02P31<br />

Three variegated clementines<br />

Kafa G., Seday U., Uysal O., and Polatöz S.<br />

Alata Horticultural Research Station Mersin (AHRS), Turkey. gucerkafa@gmail.com<br />

Three variegated clementine mutants among the M1V3 clementine plants were selected at the Alata<br />

Horticultural Research Station of Mersin. Three variegated clementine mutants obtained from budwood<br />

irradiation of 30 gray Gamma ray. Young fruit rind color attractively variegated but fruit rind color variegation<br />

fades with maturity and mature fruit rind color could easily masquerade as an ordinary clementine. The young<br />

fruit rind is striped green and orange, but mature fruit rind has ridges. Seed number is 7.5 for variegated<br />

clementine No.1, 13.07 for variegated clementine No.2, 11.94 for variegated clementine No.3 and 17.54 for<br />

control clementine plants. Fruit weight are 72.73 g for variegated clementine No.1, 69.27 g for variegated<br />

clementine No.2, 80.94 g for variegated clementine No.3 and 110.10 g for control clementine plants. Other<br />

pomological characteristics are as same variegated and control plants.<br />

S02P32<br />

New low seeded mandarins (Citrus reticulata) and lemons (Citrus lemon) selections obtained by<br />

gamma irradiation in Chile<br />

Montañola M.J., and Martiz J.<br />

Pontificia Universidad Católica de Chile (PUC), Facultad de Agronomíe e Ingeniería Forestal, Departamento de Fruticultura, Chile.<br />

mmontano@uc.cl<br />

Gamma irradiation of buds is a technique that has been successfully used in citrus breeding programs that<br />

seek to remove seeds from fruits. In 2007, Pontificia Universidad Católica de Chile <strong>CITRUS</strong> lab started a new<br />

breeding program of mandarins and lemons using this technique. Irradiated buds were grafted and plants<br />

were grown in a commercial nursery. In 2009, a field trial was established with 4500 hybrid mandarins, 1200<br />

clementines and 2500 lemons, in Pomaire, Chile (33°39’S, 71°10’W). First evaluation of fruit was made in<br />

54 - VALENCIA CONFERENCE CENTER, 18th-23rd NOVEMBER 2012

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