LIBRO-CONGRESO-CITRUS

S13P07
Potential of secondary metabolites secreted by the entomopathogenic mitosporic ascomycetes
Beauveria sp. and Metarhizium spp. for medfly Ceratitis capitata adult control.
Lozano-Tovar M.D., Garrido-Jurado I., and Quesada-Moraga E.
University of Cordoba (UCO), Agricultural and Forestry Science, Spain. z82lotom@uco.es
The Mediterranean fruit fly Ceratitis capitata (Wiedemann) is a cosmopolitan pest that feeds on a wide
range of plants. In citrus crops, it causes important direct and indirect annual costs. Medfly management is
mainly based on treatments with chemical insecticides, but there are increasing concerns on the effect of
such compounds on environment and humans. Besides, the new European Directive for the sustainable use
of pesticides promotes the non-chemical control methods with emphasis on biopesticides. This work was
performed to search for new insecticidal compounds of natural origin secreted by entomopathogenic fungi
for medfly control. Crude extracts of several B. bassiana, M. anisopliae and M. brunneum isolates and their
dialyzed and adialyzed fractions were tested on C. capitata newly emerged adults. Crude extracts from four
isolates were highly toxic, with mortalities ranging between 87 and 100% at 48 hours. In most cases, the toxic
fraction of the crude extracts was retained after dialysis 3500 da cut-off membranes. The most active fraction
was submitted to organic extraction, with methanol and buthanol fractions being highly toxic per os against
C. capitata.
S13P08
Comparison of different food attractants for fruit fly capture (Diptera: Tephritidae) in citrus
orchards in the state of São Paulo, Brazil
Rodrigues M.D.A. 1 , Raga A. 2 , Maldonado Jr W. 1 , and Barbosa J.C. 3
1 Universidade Estadual de Campinas (UNICAMP), Brazil; 2 Instituto Biológico (IB), Centro Experimental, Brazil; and 3 Universidade
Estadual Paulista (UNESP), Brazil. adalton@biologico.sp.gov.br
Anastrepha fraterculus (Wiedemann) and Ceratitis capitata (Wiedemann) are the most important fruit fly
species in Brazil. Both species are responsible for significant losses in citrus production in the State of São
Paulo. The aim of this study was to compare the efficacy of different food bait attractants for monitoring fruit
flies in IPM programmes. The experiment was conducted under completely randomized design with seven
treatments (yellow plastic McPhail traps) and four replications. The treatments were: BioAnastrepha (3%
v/v), Isca Mosca (3% v/v), Samaritá® (3% v/v), Torula® (3 tablets per trap), Milhocina® plus borax (5% v/v 3%
p/v), sugar cane molasses (7% v/v) and grape juice Aurora brand (25% v/v). The experiments were performed
in two seasons, from March until April (season 1) and from November until December 2008 (season 2), in two
orange orchards (organic and conventional systems) located in Mogi-Guaçu, SP. The number of flies captured
by each attractant was evaluated weekly during seven weeks. In total 4,327 adults of Tephritidae were
captured: 3,073 specimens of A. fraterculus and 1,258 specimens of C. capitata. Milhocina plus borax and
Torula during season 1, and Torula during season 2 were more effective to capture fruit flies. These differences
were sorted out by Tukey´s test between treatments. In the organic orchard, Torula and BioAnastrepha
captured significantly more tephritids during season 2. In general, Torula attracted more females than males.
S13P09
Evaluation of protein bait laced with various insecticides on the Queensland fruit fly (Diptera:
Tephritidae): attraction, feeding, mortality and bait persistence
Mahat K. 1 , and Drew R.A.I. 2
1 National Plant Protection Centre (NPPC), Department of Agriculture, Thimphu, Bhutan; and 2 International Centre for the Management
of Pest Fruit Flies (ICMPFF), Griffith School of Environment, Environment, Science, Engineering and Technology, Griffith University,
Nathan Campus, Queensland 4111, Australia. kiranmahat@gmail.com
Use of malathion in fruit fly protein bait sprays has raised serious concerns due to its adverse effects on
non-target organisms. This has necessitated the need to evaluate novel, reduced risk compounds. This
study evaluated the effects of spinosad, fipronil, malathion and chlorpyrifos mixed with fruit fly protein bait
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