LIBRO-CONGRESO-CITRUS

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S08 S08P10 Cloning and functional analysis of stress-responsive genes in Poncirus trifoliata Liu J.H., Wang J., Huang X.S. and Sun P.P. Huazhong Agricultural University, College of Horticulture and Forestry Science, China. liujihong@mail.hzau.edu.cn Poncirus is a widely used rootstock for citrus because it has several desirable attributes, such as cold hardiness and Citrus Tristeza Virus tolerance. However, it is not drought and salt tolerant. On the other hand, most of citrus commercial cultivars are not cold tolerant. Therefore, it is necessary to obtain novel germplasms of either rootstock or scion cultivars with enhanced stress tolerance. As a complementation to the traditional breeding, genetic transformation has been proven as an effective approach to generate plants with enhanced stress tolerance. To make this new approach applicable to citrus cultivar improvement, it is obligatory to clone and functionally characterize genes involved in stress response. During the last years we have made efforts to isolate several functional or regulatory genes involved in stress response from trifoliate orange, including PtADC, PtrABF and PtrMAPK. Function of the genes in stress tolerance was characterized using Arabidopsis or tobacco transformation. For example, overexpression of PtADC in Arabidopsis led to enhanced tolerance to osmotic stress, drought and cold stress. Interestingly, transgenic plants exhibited longer roots and smaller stomatal density. Transformation of PtrAFB and PtrMAPK in tobacco resulted in elevated drought tolerance, accompanied by the induction of stress-responsive genes and enhanced antioxidant system for ROS scavenging. These genes hold great potential for genetic transformation in citrus or trifoliate orange. S08P11 Characterization of water deficit tolerance of Poncirus trifoliata genotypes as related diversity Ben Yahmed J. 1 , Costantino G. 2 , Ben Mimoun M. 3 , Talon M. 4 , Ollitrault P. 1 , Morillon R. 1 and Luro F. 2 1 Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), Amélioration des Plantes à Multiplication Végétative, France; 2 Institut National des Recherches Agronomiques (INRA), France; 3 Institut National Agronomique de Tunisie (INAT), Arboriculture Fruitiere, Tunisie; and 4 Centro de Genómica, Instituto Valenciano de Investigaciones Agrarias (IVIA), Spain. jihene.benyahmed@gmail.com Rootstock provides better adaptation to biotic and abiotic constraints. Poncirus and its hybrids are widely used since they are tolerant to Citrus Tristeza Virus. However, when grafted, most of them are considered to be sensitive to salinity and water deficit. Diversity does exist within the Poncirus trifoliata specie but little is known regarding the behaviour variability of genotypes to abiotic constraints. The citrus diversity of 72 P. trifoliata genotypes was investigated using SSR markers. Two mayor genetic groups were clearly identified. Five genotypes, belonging to each genetic group, were then selected to investigate their properties of tolerance to water deficit. Water deficit was applied by withdrawing irrigation for 4 weeks. Physiological parameters such leaf stomatal conductance and quantum yield of PSII electron transport, soil water potential, leaf osmotic pressure and transpiration were estimated. Among genotypes, we observed that some genotypes such as’ Rubidoux’ were clearly more tolerant to water deficit than others such as ‘Pomeroy’. Interestingly, the genotypes that were sensitive belonged to one genetic group and the tolerant ones belonged to the other group. Therefore, it sounds that among the Poncirus genus, it is possible to select genotypes as parent in breeding programs, which are more tolerant to water deficit. S08P12 Screening nine citrumelo rootstocks for tolerance to drought conditions Fadli A. 1 , Beniken L. 2 , Omari F.E. 2 , Benkirane R. 1 and Benyahia H. 2 1 Ibn Tofail University (IUT), Department of Plant Science, Kenitra, Morocco; and 2 Kenitra Regional Center for Argricultural Research (CRRAK), Department of Plant Breeding and Phylogenetic Ressources Conservation, Kenitra, Morocco. a.fadli.uit@gmail.com Two months old citrumelo rootstocks groups seedlings were grown in 0.5 L plastic pots in a greenhouse at El Menzeh (CRRA Kenitra), INRA Morocco, under three moisture regimes (100%, 75% and 50% Hfc) to investigate the effects of water stress on their morphological and physiological characteristics such as plant height, number of leaves per plant, fresh matter accumulation, transpiration ratio, chlorophyll content (SPAD), soluble solids and proline content, respectively. A split plot design with ten citrus rootstocks × three treatments and 124 - VALENCIA CONFERENCE CENTER, 18th-23rd NOVEMBER 2012
three replications was used. The measurements were taken at the end of the experiment. Growth parameters (plant height, number of leaves per plant, fresh matter accumulation), stomata conductance and chlorophyll content (SPAD) decreased under water deficit (50% Hcc). Increase in water stress increased the soluble solids and proline content. It is concluded that water stress significantly (p
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THE XII INTERNATIONAL CITRUS CONGRE
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WELCOME MESSAGE On behalf of the In
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Members Dr. FLORENTINO JUSTE Direct
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Poster presentations All posters ar
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SPONSORS XII INTERNATIONAL CITRUS C
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WE TAKE CARE Managing and adding va
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Scientific Programme
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SCIENTIFIC PROGRAMME Note: Sessions
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SESSION 01: Citrus germplasm and ph
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Poster ID Title S02P01 S02P02 S02P0
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SESSION 05: BIOTECHNOLOGY Oral pres
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SESSION 08: Abiotic stress Oral pre
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SESSION 09: Postharvest physiology
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Oral ID Title S11O03 S11O04 S11O05
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Oral ID Title S12O04 S12O05 S12O06
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SESSION 15: Fungal diseases Oral pr
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Plenary Conferences PC01 The Spanis
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Drip irrigation introduced in the 8
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Global warming and its impact on cl
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egional, i.e. applied on an area-wi
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PC07 Biological control and citrus:
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W01 HLB control Convener: J.M. Bov
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mandarin cultivars such as `Fortune
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• Open: All growers have free acc
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Genetic restriction of fruit tree s
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W08 Global citrus industry collabor
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forced-air, vapor heat, cold, and i
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S01O01 Citron germplasm in Yunnan,
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citrus cultivars are interspecific
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S01P06 Diversity analysis of citrus
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Our results demonstrate that the ci
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S02O01 The triploid mandarin breedi
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S02O06 Citrus breeding in South Afr
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furanocoumarins. However, a particu
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or horticultural traits, but truene
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S02P06 Preliminary research on gene
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S02P11 Development CTV resistant ci
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S02P16 Mechanism of seedlessness in
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S02P21 Haploid and polyploid hybrid
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agronomically characterized 79 hybr
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2011. Early production was achieved
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are vigorous with nearly round-shap
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Session 03 CITRUS GENOMICS S03
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S03 of large genomic regions. Seque
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S03 S03O07 Analysis of the clementi
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S03 and recombinant DNA technology.
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S03 TFs. Some of them are MADS-box
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S03 S03P13 Fast and cost-effective
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Page 146 and 147: S05 the regulation of carotenogenes
Page 148 and 149: S05 stoma density, size and opening
Page 150 and 151: S05 S05P02 Comprehending crystallin
Page 152 and 153: S05 S05P07 D-limonene downregulatio
Page 154 and 155: S05 to this disease. Development of
Page 156 and 157: S05 tomato constitutively express L
Page 158 and 159: S05 deletor’ technology is an eff
Page 160 and 161: S05 sour orange seedlings. The embr
Page 162 and 163: S05 biodegradable makes them attrac
Page 165 and 166: S06O01 Effect of male-female intera
Page 167 and 168: S06O06 Endogenous factors affecting
Page 169 and 170: lowest levels at fruit mature stage
Page 171 and 172: (5.6% of the total citrus area) wit
Page 173: almost disappeared and total yield
Page 177 and 178: S07O01 Citrus developmental researc
Page 179 and 180: S07O06 Exploring microRNA target mo
Page 181 and 182: set of the orange varieties ‘Wash
Page 183 and 184: S07P10 Transcript accumulation of f
Page 185: Session 08 ABIOTIC STRESS S08
Page 188 and 189: S08 present study, we identified pr
Page 190 and 191: S08 S08O08 Microsprinkler irrigatio
Page 192 and 193: S08 S08P05 Role of ammonium nutriti
Page 196 and 197: S08 S08P15 Flooding and soil temper
Page 198 and 199: S08 S08P20 Orange varieties as inte
Page 200 and 201: S08 In a field experiment, the tole
Page 203 and 204: S09O01 Genome sequence of the necro
Page 205 and 206: calcofluor white (CFW) or congo red
Page 207 and 208: a continuous flow-through system (2
Page 209 and 210: experimental evidences in mandarin
Page 211 and 212: for 14 days was investigated. Chang
Page 213 and 214: S09P13 Organoleptic quality and pre
Page 215 and 216: SOD activity in the Δsod1 mutant w
Page 217 and 218: were observed. Thus, other possible
Page 219 and 220: fruits received the following treat
Page 221 and 222: 3) to evaluate different treatments
Page 223: acid, sodium carbonate and sodium m
Page 227 and 228: S10O01 Open hydroponics of citrus c
Page 229 and 230: gas exchange, foliar SPAD index and
Page 231 and 232: y citrus and consequent effects on
Page 233 and 234: strongly decreased with the applica
Page 235 and 236: strategies. The aim of this work wa
Page 237 and 238: S10P15 Efficiency of zinc (68Zn) fe
Page 239 and 240: S10P20 Phosphorus deficiency decrea
Page 241 and 242: S10P25 Foliar nutrition with macron
Page 243: S10P30 Use of plant-soil-atmosphere
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S11O01 New method of citrus graftin
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S11O06 Reduction of fruit splitting
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S11P03 Evaluation of rootstocks for
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S11P08 Production of ‘Tahiti’ a
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season and test a range of varietie
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S11P18 A phytosanitary evaluation m
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over 50% of the infested fields. Th
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S12O01 New insights into the citrus
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or Huanglongbing (HLB) and its vect
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trapping program, visual surveys, p
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S12P01 Yield loss modeling of “Ca
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amplify different geographical Huan
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affects all known citrus species an
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S12P15 Systemic insecticides are ef
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S12P20 Heat treatment of Huanglongb
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leaves. Although XCT44 produces les
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incidence of canker. Only in the ye
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lime were treated with chitosan (CH
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S12P39 Unforbidden fruits: Preventi
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S12P43 Analysis of citrus huanglonb
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S13O01 Application of the Sterile I
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(SIT) application. Both mass-trappi
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S13P03 An early step toward the dev
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S13P07 Potential of secondary metab
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climatic conditions, including Tuni
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Session 14 VIRUS AND VIRUS LIKE DIS
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S14 even if infected, an investigat
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S14 S14O08 New generation sequencin
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S14 stunting and low fruit yield an
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S14 S14P06 Molecular diversity of C
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S14 along the Ionian coast and the
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S14 S14P15 Integrated Citrus Triste
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S14 infected tree incites the psoro
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S14 Conformation Polymorphism (SSCP
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S14 infected trees, while bark scal
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S14 grafted on C. volkameriana, sou
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S15O01 The arrival of Citrus Black
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S15O06 Epidemiology of Alternaria B
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S15O11 Searching for citrus rootsto
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S15P05 Modelling of Guignardia pseu
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S15P10 Outbreak and occurrence of A
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concerns have prompted governments
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control strategies. A survey was in
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Santa Barbara, Tulare, and Ventura.
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into two groups: one dried in the s
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S16O01 The status of citrus IPM in
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S16O06 Progress toward integrated m
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was uniform under the non-UV-blocki
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S16O16 Field evaluation of some pes
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S16P01 Analysis of population trend
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S16P06 Use of oils to control the A
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stage, nymphs and eggs that were ob
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S16P16 Repellency and acceptability
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could be described by the Holling I
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S16P25 Assessment of trophic intera
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S16P30 Composition and flight activ
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2010 and 2011, the efficacy of spra
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were: esfenvalerate (0.25); deltame
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S16P44 Preliminary survey of the Gr
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Session 17 VARIETIES S17
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S17 included represent California,
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S17 S17P01 Plant growth, initial fr
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S17 S17P06 Fruit characteristics of
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S17 S17P11 Evaluation of Lemon Sele
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S17 S17P16 New tangors for Brazilia
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S17 growing areas are from Coquimbo
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S17 recorded during the previous 21
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Session 18 ROOTSTOCKS S18
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S18 sinensis × Poncirus trifoliata
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S18 lower and higher ML yields/tree
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S18 S18P03 Effect of the rootstock
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S18 S18P08 Agronomic performance of
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S18 potentially very interesting fo
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S18 favorable for the citrus indust
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S18 overfruiting and temperature du
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S18 S18P28 Tree performance and fru
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Session 20 CITRUS AND HEALTH S20
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S20 and ascorbic acid, acting indiv
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S20 dysfunction. Microarray studies
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S20 of satsuma, however, (even some
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Session 21 POSTHARVEST AND JUICE PR
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S21 determined by HPLC. Results ind
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S21 flowers of different cultivars
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S21 pallet and in the fruits) 40 di
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S21 effectiveness or completely ine
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S21 of Agriculture for agricultural
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Session 22 CITRUS ECONOMICS AND TRA
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S22 slowed down. USA is currently t
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S22 growers, this requires permanen
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S22 demand is growing due to knowle
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Author’s Index
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Beitia F. 228 Belda J.E. 290 Beldom
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del Pino A. 330, 333 Del Río J.A.
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Gush M.B. 157 Guven B. 288 Guzmán-
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Lo Presti P. 244 Locali E.C. 204 Lo
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Orce I.G. 208 Ordúz J. 239 Orea Ve
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Sela I. 107 Sela N. 133, 146, 264 S
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Xie Y.M. 87 Xin-Hua H. 28 Xingzheng
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