LIBRO-CONGRESO-CITRUS

S03
TFs. Some of them are MADS-box genes, a family of TFs involved in developmental processes whereas others
showed high partial sequence similarity restricted to specific domains but negligible outside those domains.
Transgenic Arabidopsis plants overexpressing these TFs were generated and number of leaves without abaxial
trichomes, flowering time and morphology of leaves, siliques and whole plants have been analysed. Transgenic
lines for four of the TFs showed shortening of the juvenile phase, indicating that these genes are likely
involved in determining the reproductive phase transition also in citrus. Lines which showed no phenotype
differences corresponded to TFs that have not yet been characterized in other plants and therefore they
might be novel genes that play specific roles during the juvenile-to-adult transition in citrus. This makes them
good candidates to study the specific aspects of juvenility in citrus. Deciphering the molecular mechanisms
involved in the juvenile-to-adult transition in citrus plants is crucial if we expect to reduce the generation time
for genetic studies and breeding programs. Our results represent a step forward in the knowledge of these
processes in citrus and offer the prospect of rapid transfer of information to other woody plants.
S03P09
Differential expression of proteins related to primary metabolism in ‘Moncada’ mandarin leaves
with contrasting fruit load
Muñoz-Fambuena N. 1 , Mesejo C. 1 , Agustí M. 1 , Tárraga S. 2 , Iglesias D.J. 3 , Primo-Millo E. 3 , and González-Mas M.C. 3
1 Universidad Politécnica de Valencia (UPV), Instituto Agroforestal del Mediterráneo, Spain; 2 Universidad Politécnica de Valencia
(UPV), Instituto de Biología Molecular y Celular de Plantas, Spain; 3 Instituto Valenciano de Investigaciones Agrarias (IVIA), Centro de
Citricultura y Producción Vegetal, Spain. gonzalez_mde@gva.es
A proteomic study was used to know how the primary metabolism in citrus leaves is affected by the fruit
load. To this end, we researched the differential expression of proteins related to these processes between
on-crop and off-crop ‘Moncada’ mandarin leaves. Samples were collected in November and proteins were
extracted. From 2D DIGE gel of these extracts, 33 spots related to primary metabolism were isolated: 26 spots
being associated with photosynthesis and carbohydrate metabolism, 4 spots related to Krebs cycle, 1 spot
related to pentose phosphate pathway, and 2 spots related to nutrient reservoir activity. These spots were
identified by MALDI-MS or LC-MS-MS. Between the proteins related to photosynthesis and carbohydrate
metabolism, 16 were up-expressed in off-crop leaves, such as Nadp-dependent glyceraldehyde-3-phosphate
dehydrogenase, RuBisCO large subunit, ADP-glucose pyrophosphorylase, cinnamoyl-CoA reductase, and
carbonic anhydrase; while 10 proteins were up-expressed in on-crop leaves such as NADP-dependent malic
enzyme, and 3,4-dihydroxy-2-butanone kinase. The isolated proteins related to pentose phosphate pathway
(6-phosphogluconate dehydrogenase) and to nutrient reservoir activity (Granule-bound starch synthase Ib
precursor) were up-expressed in off-crop leaves. Regarding the proteins related to Krebs cycle, only malate
dehydrogenase was up-expressed in off-crop leaves, while the other proteins were up-expressed in on-crop
leaves (citrate synthase and NADP-isocitrate dehydrogenase). According these results, the primary metabolism
seems to be more active in off-crop leaves, suggesting that fruit load inhibits the primary metabolism in
‘Moncada’ mandarin. This work has been supported by the Project INIA RTA2009-00147-C02 and Fundación
Agro Alimed.
S03P10
Proteins related to stress and redox state of Moncada mandarin leaves with contrasting fruit load
Muñoz-Fambuena N. 1 , Mesejo C. 1 , Agustí M. 1 , Tárraga S. 2 , Iglesias D.J. 3 , Primo-Millo E. 3 , and González-Mas M.C. 3
1 Universidad Politécnica de Valencia (UPV), Instituto Agroforestal del Mediterráneo, Spain; 2 Universidad Politécnica de Valencia
(UPV), Instituto de Biología Molecular y Celular de Plantas, Spain; 3 Instituto Valenciano de Investigaciones Agrarias (IVIA), Centro de
Citricultura y Producción Vegetal, Spain. gonzalez_mde@gva.es
A proteomic study was used to know more about the citrus redox state and response stress related to fruit
load. To this end, we researched expression differences of proteins related to these processes between oncrop
and off-crop ‘Moncada’ mandarin leaves. Samples were collected in November, were immediately stored
frozen at -80 ºC, and proteins were extracted in less than two weeks. From 2D DIGE gel of these extracts, 12
related spots were isolated: only 1 showed increased expression in the off-crop samples compared to on-crop
68 - VALENCIA CONFERENCE CENTER, 18th-23rd NOVEMBER 2012