LIBRO-CONGRESO-CITRUS

set of the orange varieties ‘Washington’ navel and ‘Thomson’ (Citrus sinensis).The design was a split plot in a
randomized complete block with five treatments and four replications. Flowering tissue samples were stored
in liquid N until the extraction of gibberellins and the identification and quantification of GA3. The results
show statistically significant differences (P ≤ 0.05) between treatments in the number of fruits retained on the
129 d after flowering and the percentage of final set. GA3 content ranged from 1.66 mg g -1 in the control to
20.79 mg g -1 dry weight in the high dose. The mean doses of 32.2 mg L -1 of auxins, 32.2 mg L -1 of gibberellins
and 83.2 mg L -1 of cytokinins, resulted in the largest increases in fruit set.
S07P05
Analysis of expressed proteins in the pollen tube occuring in the self-incompatible response
using mass- or single-culture system of mature pollen in citrus.
Abe A. 1 , Uchida A. 2 , Hoshino Y. 3 , Sakakibara Y. 1 , Suiko M. 1 , and Kunitake H. 1
1 University of Miyazaki (UOM), The Graduate School of Agriculture, Japan; 2 University of Miyazaki (UOM), The Interdisciplinary
Graduate School of Agriculture and Engineering, Japan; and 3 Hokkaido University (HU), Field Science Center for Northern Biosphere,
Japan. ae11003@student.miyazaki-u.ac.jp
To analyze protein expression of pollen tubes having self-incompatible reactions in hyuganatsu (Citrus
tamurana), mature pollen was cultured and treated with crude extracts from styles using the mass or single
liquid culture system and pollen tubes were collected for proteome analysis. The relative expression of
each identified protein was quantified by Imager and revealed as a value relative to the pollen grain. Eleven
identified proteins were remarkably up-regulated (above 1.2-fold) or down-regulated (under 0.8-fold) in the
treatment with crude extract from self-pollinated styles. Seven of these proteins were predicted to be related
to the SI reaction between the reported function of these seven proteins. In this study, F-box protein was
identified as showing minimum expression in the treatment with extract from styles of wild-type hyuganatsu.
The S-locus gene product expressed in pollen has been identified as an F-box protein in Solanaceae and
Rosaceae. It is not clear whether a mechanism similar to that in Solanaceae and Rosaceae exists in the SI
reaction in hyuganatsu, however, the reduced expression of F-box protein may induce an SI reaction.
S07P06
Hormone complex and micronutrients in production and fruit quality of ‘Valencia’ sweet orange
Galván J.J., López N., and Tolentino A.
UAAAN (UA), Horticultura, Saltillo. juan.galvan@uaaan.mx, doctorgalvanluna@hotmail.com
A complex hormone (Biozyme ® TF) was applied with micronutrients (Zinc poliquel and multi) at different
concentrations and we evaluated the effects on both quality and performance of the fruit of ‘Valencia’ sweet
orange in 20 year-old trees. Tree were located near the Eugenia Huerta Montemorelos Monterrey road Km
66, Gil de Leyva Montemorelos Nuevo Leon, Mexico, between 25° 11’ 24” north latitude and 99° 41’ 33” west
longitude of Greenwich, with an altitude of 423 m and a rainfall of 600 to 1.000 mm. The prevailing weather
was semi-warm with an annual average temperature of 18º C to 22º C and humid with rains intermediate in
summer and winter. The variables evaluated were fruit weight (FW), equatorial diameter (DE), polar diameter
(DP), thickness of the peel (GC), fruit firmness (F), % juice content (CJ), volume of juice (VJ), weight of juice (PJ),
º Brix (GB), pH of the juice, citric acid % (AC), vitamin C, color ratio L * a * b * and number of seeds per fruit. The
results show that treatment of Biozyme Poliquel Zn), positively affected the majority of the variables evaluted.
S07P07
Effects of Triclopyr (3,5,6-trichloro-2-pyridyloxyactetic acid) applications on fruit quality of
SRA63, SRA85, SRA88 and SRA92 clementines
Zurru R. 1 , Deidda B. 1 , Dessena L. 2 , and Mulas M. 2
1 AGRIS-Sardegna (AGRIS), Department of Wood and Fruit Tree Research, Italy; and 2 University of Sassari (DIPNET), Department of the
Nature and Land Sciences, Italy. mmulas@uniss.it
In order to improve the yield and quality of clementine fruit, many treatments with hormonal compounds
were carried out during the last 8 years in the south of Sardinia (Italy). The main objectives of the research
XII INTERNATIONAL CITRUS CONGRESS 2012 - 111
S07