LIBRO-CONGRESO-CITRUS

S20
and ascorbic acid, acting individually or in concert. Recent human clinical studies suggest that citrus bioactives
provide most of their beneficial effects via processes targeting one or more steps of chronic inflammation
processes. Long term inflammation is considered to be a key element in the development of many chronic
diseases: cardiovascular diseases, metabolic syndrome, osteoporosis, dementia and some forms of cancer.
Citrus phytochemicals appear to act through their metabolites by modulating complex biochemical pathways,
signalling pathways, or differential expression of specific disease-related genes. The establishment of healthbeneficial
‘causes and effects’ associated with consumption of citrus fruit and juice products has been the
focus of a significant number of recent clinical studies. A review of human clinical studies, and relevant
supportive evidence, will be reported.
S20O04
Effects of a long-term grapefruit juice consumption on vascular protection and bone metabolism:
a controlled randomized cross-over study in post-menopausal women to determine the specific
role of naringin
Habauzit V1 , Verny M.A. 2 , Milenkovic D. 2 , Pickering G. 1 , Duale C. 1 , Bayle D. 2 , Thien S. 2 , Rambeau M. 2 , Mazur A. 2 , Horcajada
M.N. 2 , Dubray C. 1 , and Morand C. 2
1Centre Investigation Clinique, CIC-CPC INSERM 501, Centre Hospitalier Universitaire, Clermont-Ferrand, France; and 2INRA, UMR
1019, Unité de Nutrition Humaine, France. christine.morand@clermont.inra.fr
The consumption of some specific polyphenol-rich foods (cocoa, green tea or soya products) has been
associated with improvement in several intermediate markers of risk related to cardiovascular disease (CVD)
and osteoporosis. Citrus fruits also constitute dietary polyphenol-rich sources; however clinical evidence of
the role of polyphenols in citrus health effects is less consistent.
We aimed at 1) characterizing the effect of a long-term consumption of grapefruit juice on both vascular
and bone protections in humans and 2) assessing the specific role of its main bioactive phenolic (naringin)
in the observed effects. Fifty-two healthy post-menopausal women (non-smoking, aged 50-65 years, 3-10
years since menopause, waist size > 88 cm) were enrolled in a randomized, controlled cross-over trial. For
two periods of 6 months, subjects consumed 340 ml/d of either a grapefruit juice (providing 100 mg naringin
per serving) or a control beverage mimicking the composition of juice but without naringin. Beyond the
monitoring of the effect of treatments on conventional risk factors for CVD, the main outcomes were the
evaluation of endothelium-dependent vasoreactivity, in both macro- and microcirculation (brachial FMD
and PAT signal), and arterial stiffness (carotid-femoral PWV). Regarding bone metabolism, the main outcome
measured was impact on bone mineral density at multiple sites. The statistical analyses of the obtained data
are currently going on.
S20O05
Effect of technological treatments on the bioavailability of flavanones from orange
Vallejo F. 1 , Tomás-Navarro M. 1 , Carbonell J.V. 2 , Navarro J.L. 2 , and Tomás-Barberán F.A. 1
1Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC), Ciencia y Tecnología de Alimentos, España; and 2Instituto de
Agroquímica y Tecnología de Alimentos (IATA-CSIC), Ciencia de los Alimentos, España. fvallejo@cebas.csic.es
The effect of solubility and concentration of citrus juice flavanones on their bioavailability was evaluated.
Fresh squeezed orange juice (FS) and orange juice produced by two different technological processes (High
Pressure Homogenization (HPH) and Pasteurization (P)) were compared. Dissimilar flavanone precipitations
among treatments were observed. Thus, HPH treatment kept in suspension through soluble part higher
amount of flavanones increasing therefore their bioavailability. The cultivars studied were ‘Ortanique’ and
‘Lane Late’. The study was carried out in healthy volunteers (n=18) following the administration of 400 mL of
orange juices and 24-h urine collection. The determination of the total amount of flavanones present in the
supernatant and pellet of juices was measured by HPLC-ESI-MSn. The total excretion of Phase II metabolites
in all volunteers was quantified by UPLC-QqQ using the relevant standard metabolites produced by organic
synthesis (glucuronidated and sulfonated flavanone metabolites).
344 - VALENCIA CONFERENCE CENTER, 18th-23rd NOVEMBER 2012