LIBRO-CONGRESO-CITRUS

assessed. Fruits were collected when ripe. Small embryos isolated from undeveloped seeds were cultured on
Murashige and Skoog culture media supplemented with 1mg/L of gibberillic acid. Triploid plantlets selected
by flow cytometry were grafted onto `Troyer´ citrange at INRA domain with spacing 6 x 3 meters. Two triploid
mandarins, `Aya´ and `Hana´, were identified as the best triploid hybrids showing commercial interest. These
clones were described and registered in the official catalogue as new mandarins cultivars.
S17P30
Mutation breeding for seedless cultivars in Argentina
Garavello M. 1 , Anderson C. 1 , Prina A. 2 , and Martinez A. 2
1 Instituto Nacional de Tecnologia Agropecuaria (INTA), Concordia, Entre Ríos, Argentina; and 2 instituto de Genética “Edwald A. Favret”
(IGEAF - INTA), Castelar, Buenos Aires, Argentina. mgaravello79@gmail.com
For many years the application of irradiation techniques on citrus buds has been used in producing lowseeded
selections from existing high quality citrus cultivars. An irradiating program began in 2008 at
Concordia Experiment Station using X-rays for the elimination of seeds in commercially important cultivars.
The irradiations were carried out at the Institute of Genetics belonging to the National Agricultural Research
Center of INTA in Castelar, BsAs. The first step was to adjust the dose and exposure time on material of
different species of citrus. Doses of 2 and 4 kr on grapefruit hybrids, and doses of 2, 4, 6, 8 and 10 kr on
`Bonelli´ lemon (Citrus limon), `Roble´ sweet orange (C. sinensis) and `Willow leaf´ mandarin (C. deliciosa)
were used. The irradiated material was top-worked on sweet orange trees in a high density plot. The
doses were 2 kr for mandarins, and 4 kr for lemons, grapefruits and oranges. There were two subsequent
treatments on the varieties, one in November of 2008 and another in March of 2009. The selections
obtained were: two of sweet orange (0-1 seed/fruit), two of lemon (2-3 seeds/fruit) and one of grapefruit
(5 seeds/fruit). These selections were budded in a nursery to assess their stability, production and other
characteristics of interest.
XII INTERNATIONAL CITRUS CONGRESS 2012 - 319
S17