3. FOOD ChEMISTRy & bIOTEChNOLOGy 3.1. Lectures

Chem. Listy, 102, s265–s1311 (2008) Food Chemistry & Biotechnology
3
1 – Aeration
2 – Stock bottle with mineral medium
3 – Pump
1 - Aeration
4 – Flow Cell FC 81
2 - Stock bottle with mineral medium
Fig. 3 - 2. Pump Scheme of the FC 81 Flow Cell connection during the
experiment 4 - Flow cell FC 81
5
5
1
2 3
1 – Cover Glass
1 - Cover 2 – Slide glass
4 – Space for medium flow
2 - Slide 3 – Cellophane 5 – Parts of the flow cell
3 - Cellophane
Fig. 3. Cross-section of the FC 81 Flow Cell
4 - Space for medium flow
5 - Parts of the flow cell
Results
C h a r a c t e r o f C e l l E n v e l o p e s o f
T r i c h o s p o r o n C u t a n e u m
The results obtained by MATH method are summarized
in Table I. The cell envelopes of the population Trichosporon
cutaneum grown in the presence of filter paper had the most
4
4
2
Table II
Influence of shear stress on the cellophane colonization and object morphology changes
5
5
1
s655
Table I
Hydrophobity of cell envelopes in dependence on using different
types of cellulosic substrates as sole of carbon
Cellulosic substrate Cell hydrofobity [%]
filter paper 83.8
cellophane 51.8
carboxymethylcellulose 76.4
hydroxyethylcellulose 79.3
Sigmacell Type 101 9.6
Fig. 4. Cells of Trichosporon cutaneum attached on cellophane
(phase contrast)
hydrophobic character (83.8 %), whereas the population cultivated
on Sigmacell had very hydrophilic cell surfaces. The
hydrophobity of cellophane stripes (95.6 %) was determined
at Department of Polymers, ICT Prague. We wanted to choose
cells with similar surface characters as the cellophane
stripes. Filter paper could not be used for biomass preparation
because of low biomass concentration. For this reason
HEC was used for following experiments.
I n f l u e n c e o f S h e a r S t r e s s o n
C e l l o p h a n e C o l o n i z a t i o n
Low flow rates of medium are less favourable for
the cellophane colonization (Table II). Also small areas
Flow rate [ml min –1 ] Colonized area [%] Objects in field Area [μm 2 ] Lenght [μm] Width [μm] Elongation*
2 4.86 12 180.98 27.92 5.94 1.76
4 4.42 12 176.64 24.00 6.33 1.81
6 16.07 27 194.27 29.56 5.40 1.76
8 8.49 40 80.42 15.02 5.01 1.69
10 4.25 21 97.32 15.69 5.50 1.74
15 2.38 15 86.32 15.79 5.25 1.97
20 6.08 24 117.22 20.90 5.65 2.18