3. FOOD ChEMISTRy & bIOTEChNOLOGy 3.1. Lectures

Chem. Listy, 102, s265–s1311 (2008) Food Chemistry & Biotechnology
of functioning in reflexion, wolfram lamp, scanning method:
zig zag, λ = 550 nm.
Results and Discussions
Workings in the described condition there are presented
the flavonoid concentrations in leaves, respectively in flowers
from Melampyrum cristatum L.
After the photodensitometric evaluation for the Melampyrum
cristatum leaves, the sample which is more concentrated
in flavonoides, the densitogram from Fig. 4 was
obtained. The height of the drops in densitogram, the low
flavonoide concentration.
Fig. 1. Mass spectrum of methanolic unhydrolysed extract
from Melampyrum cristatum l. leaves
Table I
Concentration of methanolic unhydrolyzed extract from Melampyrum cristatum L. leaves
Compound nr. Identification Confirmation Concentration The quantity
UV MS in extract [μg ml –1 ] in vegetable drug [mg g –1 ]
Isoquercitrin 9 yes yes 60.838 0.30419
Rutozid 10 yes yes 3.715 0.01858
Luteolina 16 yes yes 153.390 0.76695
Apigenina 18 yes yes 15.195 0.07598
Table II
Concentration of methanolic hydrolysed extract from Melampyrum cristatum L. leaves
Compound nr. Identification Confirmation Concentration The quantity
UV MS in extract [μg ml –1 ] in vegetable drug [mg g –1 ]
Quercetol 14 yes yes 3.380 0.01690
Luteolina 16 yes yes 131.092 0.65546
Kaempferol 17 yes yes 1.439 0.00720
Apigenina 18 yes yes 11.235 0.05618
Table III
Concentration of methanolic unhydrolysed extract from Melampyrum cristatum L. flowers
Compound nr.
Identification Confirmation Concentration The quantity
UV MS in extract [μg ml –1 ] in vegetable drug [mg g –1 ]
Isoquercitrin 9 yes yes 46.930 0.23465
Rutozid 10 yes yes 2.876 0.01438
Luteolina 16 yes yes 140.680 0.70340
Apigenina 18 yes yes 18.190 0.09095
s740
Fig. 2. Mass spectrum of methanolic hydrolysed extract from
Melampyrum cristatum l. leaves
Fig. 3. Mass spectrum of methanolic unhydrolysed extract
from Melampyrum cristatum l. flowers