3. FOOD ChEMISTRy & bIOTEChNOLOGy 3.1. Lectures

Chem. Listy, 102, s265–s1311 (2008) Food Chemistry & Biotechnology
Standard curve of β-carotene are shown in Fig. 1. We
prepared standard solution from 25 µg ml –1 to 400 µg ml –1 for
construction of a standard curve in our study.
We were interested to quantification of real value of
β-Carotene in oil matrix gelatin capsule vitamin supplement.
We were conducting extraction process at water bath at 30 °C
for 2 min and at ambient temperature. The samples were
completely extracted 6 times after 30 minute extraction using
n-hexane.
Fig. 2. Chromatograms of a gelatin capsule vitamin supplements
Peak 3 were positively identified like β-carotene. Each
sample was measured five times (Table II).
Result of our study shows 5.83 ± 0.02 mg per capsule
β-carotene in examined samples.
s576
Table II
Content of β-carotene in a gelatin capsule vitamin supplement
Sample Average peak area Content of β-caroten
[mV s –1 ] in tablet [mg]
1 52.00 5.804
2 51.25 5.700
3 53.30 5.986
4 52.45 5.867
5 53.40 5.999
Average 52.48 5.83 ± 0.020
Conclusions
This study shows that the gelatin capsule vitamin supplement
analyzed here contains 5.83 ± 0.02 mg of β-carotene.
Producer declares 6.00 mg in each capsule. Must be checked
it, if producer has a right vehicle for mixture of β-carotene
and blueberry extract, because we have verified our method
with standard addition. Finally we can conclude that HPLC
with ECD method have shown to be acceptable for determination
and quantification of β-carotene in a gelatin capsule
vitamin supplements.
REFEREnCES
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M. C., Fernandez Ruiz V., Lopez Saenz de Tejada M.: J.
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4. Ferruzzi M., Mario G.: J. Anal. Biochem. 256, 74
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