3. FOOD ChEMISTRy & bIOTEChNOLOGy 3.1. Lectures

Chem. Listy, 102, s265–s1311 (2008) Food Chemistry & Biotechnology
Table I
Production of biomass and beta-carotene by stressed red yeasts in laboratory flasks
R. glutinis biomass R. glutinis Beta-carotene S. roseus biomass S. roseus Beta-carotene
control 11.1 0.18 11.5 0.16
2 mM peroxide/InO 10.6 0.17 10.6 0.26
2% salt/ InO 11.8 0.38 11.6 0.05
2% salt/ InO/
10.1 0.56 11.7 0.17
5 mM peroxide/prod.
2 mM peroxide/InO/
5 mM peroxide/prod.
to production media. Liquid whey exhibited negative effect.
Also potato extract added into InO II led to increased betacarotene
production, while biomass yield was lower.
Rhodotorula rubra is poor producer of carotenoids
when compared with other strains. However, cultivation in
presence of potato extract in InO II combined with salt stress
in production medium led to the highest biomass as well as
beta-carotene production observed yet.
Sporobolomyces roseus exhibited substantial changes
in biomass : carotene ratio dependent on whey addition. Substantial
biomass decrease in presence of lyophillized whey
in InO II (under 5 g dm –3 ) was accompanied by very high
beta-carotene yield.
In flasks combined stress led to induction of carotenoid
production in all studied strains; 3 × increase of beta-carotene
(R.glutinis) was obtained. Addition of stress factor into inoculation
medium induced slight increase of biomass production
(salt - R.glutinis) and beta-carotene production (R. glutinis).
Conclusions
Changes in medium composition can lead to substantial
changes in biomass as well as carotenoid production. Waste
10.0 0.29 10.0 0.29
s616
substrates can be used as medium component, which can in
particular strains and conditions induce carotenoid as well
as biomass production. Thus, waste substrates could be used
industrially for carotenoid-rich biomass production.
Predominantly strain Rhodotorula glutinis CCY 20-2-26
can be used for industrial production of carotenoid-rich biomass
using processed waste substrates and/or mild physiological
stress.
This work has been supported by project IAA400310506
of Grant Agency of the Academy of Sciences of the Czech
Republic.
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