Visit our Expo - Redox and Inflammation signaling 2012

More documents

Recommendations

Info

A new antisense transcript is involved in the genomic imprinting control at the H19/IGF2 locus Nathalie Berteaux 1 , Nicole Aptel 2 , Thierry Dugimont 1 , Guy Cathala 2 , Céline Janton 1 , Jean Coll 3 , Hubert Hondermarck 1 , Jean-Jacques Curgy 1 , Thierry Forné 2 and Eric Adriaenssens 1 . 1 ERI-8 INSERM, UPRES-EA 1033, IFR 118, USTL, 59655 Villeneuve d’Ascq cedex, France ; 2 Institut de Génétique Moléculaire, UMR 5535 CNRS, Université Montpellier II, IFR 122, Montpellier, France ; 3 UMR 8527 CNRS, IBL, Institut Pasteur, Lille, France. E-mail : eric.adriaenssens@univ-lille1.fr Natural antisense transcripts have been implicated in many aspects of eucaroytic gene expression including genomic imprinting and RNA interference. Our group has identified a new antisense transcript at the human and mouse H19/IGF2 locus, that we called 91H. This locus belongs to an imprinting domain located in human chromosome 11p15.5 (homologue to the mouse distal chromosome 7). H19 is expressed from the maternal allele and Igf-2 is paternally expressed. Both genes share common regulatory sequences including the H19 5’ differentially methylated domain ICR (Imprinting Control Region) and the 3’ downstream enhancer sequences. In human, 91H is an 120 kb-long transcript, antisense to H19, spanning the entire H19 transcribed region and extending 69Kb and 39 kb downstream and upstream, respectively. This includes the ICR and the 3’ enhancer regions but not the IGF2 transcription unit. The promoter region is contained in the first intron of the MrpL23 gene (the H19 3’neighbouring gene). In addition, we show that in mouse 91H is a biallelic transcript. We demonstrate that this antisense RNA is a nuclear and short-lived transcipt in human and mouse normal cells, but we observe its stabilization and accumulation in human breast epithelial cells. Consistently, an in vivo study confirms these data revealing a 91H overexpression in breast cancer biopsies. Furthermore, the developmental expression of 91H is very similar to those of the H19 RNA. Knock-down of 91H by RNA interference leads to loss of silencing of the Igf-2 gene on the paternal chromosome and a down-regulation of Igf-2 expression. We investigate the effect of 91H on DNA methylation in ICR and differentially methylated region of this locus but also in heterochromatin formation leading to gene silencing. Our results indicate that 91H plays a key role in genomic imprinting at the H19/IGF2 locus and we discuss the role of long intergenic antisense transcript in chromatin remodelling and imprinting maintenance. Publications: 1: Berteaux N, Lottin S, Monte D, Pinte S, Quatannens B, Coll J, Hondermarck H, Curgy JJ, Dugimont T, Adriaenssens E. H19 mRNA-like noncoding RNA promotes breast cancer cell proliferation through positive control by E2F1. J Biol Chem. 2005, 280:29625-36. 2: Lottin S, Adriaenssens E, Berteaux N, Lepretre A, Vilain MO, Denhez E, Coll J, Dugimont T, Curgy JJ. The human H19 gene is frequently overexpressed in myometrium and stroma during pathological endometrial proliferative events. Eur J Cancer. 2005, 41:168-77. 3: Berteaux N, Lottin S, Adriaenssens E, Van Coppenolle F, Leroy X, Coll J, Dugimont T, Curgy JJ. Hormonal regulation of H19 gene expression in prostate epithelial cells. J Endocrinol. 2004, 183:69-78. - 24 -
4: : Lottin S, Vercoutter-Edouart AS, Adriaenssens E, Czeszak X, Lemoine J, Roudbaraki M, Coll J, Hondermarck H, Dugimont T, Curgy JJ. Thioredoxin post-transcriptional regulation by H19 provides a new function to mRNA-like non-coding RNA. Oncogene. 2002, 21:1625-31. 5: Yazidi-Belkoura IE, Adriaenssens E, Vercoutter-Edouart AS, Lemoine J, Nurcombe V, Hondermarck H. Proteomics of breast cancer: outcomes and prospects.Technol Cancer Res Treat. 2002, 1:287-96. 6: Lottin S, Adriaenssens E, Dupressoir T, Berteaux N, Montpellier C, Coll J, Dugimont T, Curgy JJ. Overexpression of an ectopic H19 gene enhances the tumorigenic properties of breast cancer cells. Carcinogenesis. 2002, 23:1885-95. 7 Hondermarck H, Dolle L, El Yazidi-Belkoura I, Vercoutter-Edouart AS, Adriaenssens E, Lemoine J. Functional proteomics of breast cancer for signal pathway profiling and target discovery. J Mammary Gland Biol Neoplasia. 2002, 7:395-405. 8: Adriaenssens E, Lottin S, Berteaux N, Hornez L, Fauquette W, Fafeur V, Peyrat JP, Le Bourhis X, Hondermarck H, Coll J, Dugimont T, Curgy JJ. Cross-talk between mesenchyme and epithelium increases H19 gene expression during scattering and morphogenesis of epithelial cells. Exp Cell Res. 2002, 275:215-29. 9: Adriaenssens E, Lemoine J, El Yazidi-Belkoura I, Hondermarck H. Growth signaling in breast cancer cells: outcomes and promises of proteomics. Biochem Pharmacol. 2002, 64:797-803. 10: Adriaenssens E, Lottin S, Dugimont T, Fauquette W, Coll J, Dupouy JP, Boilly B, Curgy JJ. Steroid hormones modulate H19 gene expression in both mammary gland and uterus. Oncogene. 1999, 18:4460-73. 11: Adriaenssens E, Dumont L, Lottin S, Bolle D, Lepretre A, Delobelle A, Bouali F, Dugimont T, Coll J, Curgy JJ. H19 overexpression in breast adenocarcinoma stromal cells is associated with tumor values and steroid receptor status but independent of p53 and Ki-67 expression. Am J Pathol. 1998, 153:1597-607. 12: Dugimont T, Montpellier C, Adriaenssens E, Lottin S, Dumont L, Iotsova V, Lagrou C, Stehelin D, Coll J, Curgy JJ. The H19 TATA-less promoter is efficiently repressed by wild-type tumor suppressor gene product p53. Oncogene. 1998, 16:2395-401. - 25 -
Page 1 and 2: - 1 - Luxembourg, January 25th to 2
Page 3 and 4: Table of content PREFACE ..........
Page 5 and 6: Preface In 1998, we organized the f
Page 7 and 8: Acknowledgments This meeting has be
Page 9 and 10: Group B (15h00 - 17h00) Session V.
Page 11 and 12: - 11 - Exhibition
Page 13 and 14: Visit our Expo (in alphabetical ord
Page 15 and 16: Thursday January 26th, 2006 (Early
Page 17 and 18: Thursday January 26th, 2006 (Evenin
Page 19 and 20: Regulation of inflammation and gluc
Page 21 and 22: Saturday January 28th, 2006 (Early
Page 23: Oral presentations (by alphabetical
Page 27 and 28: the B-Raf/mitogen-activated/extrace
Page 29 and 30: Albertini MC, Accorsi A, Citterio B
Page 31 and 32: AP-1-dependent gene expression in s
Page 33 and 34: Jaks and cytokine receptors - an in
Page 35 and 36: The Role of Met-Gab1 Signalling in
Page 37 and 38: The mammalian tumor suppressor Scri
Page 39 and 40: SIGNAL TRANSDUCTION BY STRESS-ACTIV
Page 41 and 42: Title to be announced Caroline Dive
Page 43 and 44: Mechanisms of DNA methylation in ma
Page 45 and 46: Blocking the "4 Integrin-Paxillin I
Page 47 and 48: Regulation of NF-kB-dependent trans
Page 49 and 50: The immunomodulator AS101 induces g
Page 51 and 52: Signaling pathways leading to the a
Page 53 and 54: Boute, N., Jockers, R. and Issad, T
Page 55 and 56: Jespsen JS, Sorensen MD and Wengel
Page 57 and 58: 8. Nishikawa, H., Kawai, K., Tanaka
Page 59 and 60: 9) Proteome analysis of rat pancrea
Page 61 and 62: MicroRNA is an anti-apoptotic facto
Page 63 and 64: Resveratrol inhibits phorbol ester-
Page 65 and 66: Cross-talk between angiotensin II a
Page 67 and 68: Multiple role of histamine H 1 rece
Page 69 and 70: PI3K Targeting by the Beta-GBP Cyto
Page 71 and 72: D.P. Chopra, R.E. Menard, J. Janusz
Page 73 and 74: [2] Meijer, L., Flajolet, M. and Gr
Page 75 and 76:
[4] Niemand, C., Nimmesgern, A., Ha
Page 77 and 78:
Oncogenic signaling by mutant p53 M
Page 79 and 80:
activity of the Peroxisome Prolifer
Page 81 and 82:
Restauration of SHIP-1 activity in
Page 83 and 84:
Hypoxia Signaling. Impact on Tumor
Page 85 and 86:
5. Sokolov EI, Zykov KA, Pukhalsky
Page 87 and 88:
HOW ANTITOXIC AND ANTICANCER AGENTS
Page 89 and 90:
Listeria monocytogenes induced Rac1
Page 91 and 92:
Epigenetic Regulation of Stem Cell
Page 93 and 94:
Survey on in vitro cell death induc
Page 95 and 96:
Distinct roles of IRF-3 and IRF-7 i
Page 97 and 98:
Redox-Sensitive Transcription Facto
Page 99 and 100:
Epigenic control of MHC2TA transcri
Page 101 and 102:
Attenuation of MSK1-driven NF-kB ge
Page 103 and 104:
Talking to chromatin: Silencers Sil
Page 105 and 106:
Ubiquitin ligase Smurf1 controls os
Page 107 and 108:
Workshop presentations (by alphabet
Page 109 and 110:
Ambion MicroRNAs (miRNAs) are small
Page 111 and 112:
Organizer: BIOBASE GmbH Date: Janua
Page 113 and 114:
Reliable and efficient gene Knock-d
Page 115 and 116:
Exploring ubiquitin signaling with
Page 117 and 118:
Poster presentations Poster are cla
Page 119 and 120:
Session I : protein domains Poster
Page 121 and 122:
Page 123 and 124:
Page 125 and 126:
Notes Notes - 125 -
Page 127 and 128:
Session II. Receptor signaling and
Page 129 and 130:
Session II : Receptor signaling and
Page 131 and 132:
Page 133 and 134:
Page 135 and 136:
Page 137 and 138:
Page 139 and 140:
Page 141 and 142:
Page 143 and 144:
Page 145 and 146:
Page 147 and 148:
Page 149 and 150:
Page 151 and 152:
Page 153 and 154:
Page 155 and 156:
Page 157 and 158:
Page 159 and 160:
Page 161 and 162:
Page 163 and 164:
Page 165 and 166:
Page 167 and 168:
Page 169 and 170:
Page 171 and 172:
Page 173 and 174:
Page 175 and 176:
Page 177 and 178:
Page 179 and 180:
Page 181 and 182:
Notes Notes - 181 -
Page 183 and 184:
Session III. Protein kinase cascade
Page 185 and 186:
Session III : Protein kinase cascad
Page 187 and 188:
Page 189 and 190:
Page 191 and 192:
Page 193 and 194:
Page 195 and 196:
Page 197 and 198:
Page 199 and 200:
Page 201 and 202:
Page 203 and 204:
Page 205 and 206:
Page 207 and 208:
Page 209 and 210:
Page 211 and 212:
Page 213 and 214:
Page 215 and 216:
Page 217 and 218:
Page 219 and 220:
Page 221 and 222:
Page 223 and 224:
Page 225 and 226:
Page 227 and 228:
Page 229 and 230:
Page 231 and 232:
Page 233 and 234:
Page 235 and 236:
Page 237 and 238:
Page 239 and 240:
Page 241 and 242:
Page 243 and 244:
Page 245 and 246:
Page 247 and 248:
Page 249 and 250:
Page 251 and 252:
Page 253 and 254:
Page 255 and 256:
Notes Notes - 255 -
Page 257 and 258:
Session IV. Protein kinase inhibito
Page 259 and 260:
IV. Protein kinase inhibitors: insi
Page 261 and 262:
IV. Protein kinase inhibitors: insi
Page 263 and 264:
Notes - 263 -
Page 265 and 266:
- 265 -
Page 267 and 268:
V. Phosphatases as key cell signali
Page 269 and 270:
Page 271 and 272:
Page 273 and 274:
Notes - 273 -
Page 275 and 276:
- 275 -
Page 277 and 278:
VI. Proteasome degradation pathways
Page 279 and 280:
VI. Proteasome degradation pathways
Page 281 and 282:
Session VII. Stem cell specific cel
Page 283 and 284:
Notes - 283 -
Page 285 and 286:
VIII. Inflammation specific signali
Page 287 and 288:
Page 289 and 290:
Page 291 and 292:
Page 293 and 294:
Page 295 and 296:
Page 297 and 298:
Page 299 and 300:
Page 301 and 302:
Page 303 and 304:
Page 305 and 306:
Page 307 and 308:
Page 309 and 310:
Page 311 and 312:
Page 313 and 314:
Page 315 and 316:
Page 317 and 318:
Page 319 and 320:
Page 321 and 322:
Notes - 321 -
Page 323 and 324:
- 323 -
Page 325 and 326:
Session IX. Novel compounds targeti
Page 327 and 328:
Page 329 and 330:
Page 331 and 332:
Page 333 and 334:
Session X : Cell death in cancer -
Page 335 and 336:
Session X : Cell death in cancer Po
Page 337 and 338:
Page 339 and 340:
Page 341 and 342:
Page 343 and 344:
Page 345 and 346:
Page 347 and 348:
Page 349 and 350:
Page 351 and 352:
Page 353 and 354:
Page 355 and 356:
Page 357 and 358:
Page 359 and 360:
Page 361 and 362:
Page 363 and 364:
Page 365 and 366:
Page 367 and 368:
Page 369 and 370:
Page 371 and 372:
Page 373 and 374:
Page 375 and 376:
Page 377 and 378:
Page 379 and 380:
Page 381 and 382:
Page 383 and 384:
Page 385 and 386:
Page 387 and 388:
Page 389 and 390:
Page 391 and 392:
Page 393 and 394:
Page 395 and 396:
Page 397 and 398:
Page 399 and 400:
Page 401 and 402:
Page 403 and 404:
Page 405 and 406:
Page 407 and 408:
Page 409 and 410:
Page 411 and 412:
Page 413 and 414:
Page 415 and 416:
Page 417 and 418:
Page 419 and 420:
Page 421 and 422:
Page 423 and 424:
Page 425 and 426:
Page 427 and 428:
Page 429 and 430:
Page 431 and 432:
Page 433 and 434:
Page 435 and 436:
Page 437 and 438:
Page 439 and 440:
Page 441 and 442:
Page 443 and 444:
Notes Notes - 443 -
Page 445 and 446:
Session XI : Cell death and cardiov
Page 447 and 448:
Session XI: Cell death and cardiova
Page 449 and 450:
Page 451 and 452:
Page 453 and 454:
Page 455 and 456:
Page 457 and 458:
Notes - 457 -
Page 459 and 460:
Session XII : Cell death and neurod
Page 461 and 462:
Page 463 and 464:
Page 465 and 466:
Page 467 and 468:
Page 469 and 470:
Page 471 and 472:
Page 473 and 474:
Page 475 and 476:
Page 477 and 478:
Page 479 and 480:
Page 481 and 482:
Page 483 and 484:
Page 485 and 486:
Notes - 485 -
Page 487 and 488:
- 487 -
Page 489 and 490:
Session XIII : Cell signaling pathw
Page 491 and 492:
Page 493 and 494:
Page 495 and 496:
Page 497 and 498:
Page 499 and 500:
Page 501 and 502:
Notes Notes - 501 -
Page 503 and 504:
Session XIV : Transcriptional and t
Page 505 and 506:
Page 507 and 508:
Page 509 and 510:
Page 511 and 512:
Page 513 and 514:
Page 515 and 516:
Page 517 and 518:
Page 519 and 520:
Page 521 and 522:
Page 523 and 524:
Page 525 and 526:
Page 527 and 528:
Page 529 and 530:
Page 531 and 532:
Page 533 and 534:
Page 535 and 536:
Page 537 and 538:
Page 539 and 540:
Page 541 and 542:
Page 543 and 544:
Page 545 and 546:
Page 547 and 548:
Page 549 and 550:
Page 551 and 552:
Page 553 and 554:
Page 555 and 556:
Page 557 and 558:
Page 559 and 560:
Page 561 and 562:
Page 563 and 564:
Page 565 and 566:
Page 567 and 568:
Page 569 and 570:
Page 571 and 572:
Page 573 and 574:
Page 575 and 576:
Page 577 and 578:
Page 579 and 580:
Page 581 and 582:
Page 583 and 584:
Notes Notes - 583 -
Page 585 and 586:
Session XV : Reactive oxygen specie
Page 587 and 588:
Page 589 and 590:
Page 591 and 592:
Notes - 591 -
Page 593 and 594:
Session XVI : Chemopreventive agent
Page 595 and 596:
Page 597 and 598:
Page 599 and 600:
Page 601 and 602:
Page 603 and 604:
Page 605 and 606:
Page 607 and 608:
Session XVII : Cell signaling in he
Page 609 and 610:
Page 611 and 612:
Page 613 and 614:
Page 615 and 616:
Page 617 and 618:
Page 619 and 620:
Page 621 and 622:
Page 623 and 624:
Page 625 and 626:
Page 627 and 628:
Page 629 and 630:
Page 631 and 632:
Page 633 and 634:
Page 635 and 636:
Page 637 and 638:
Page 639 and 640:
Page 641 and 642:
Page 643 and 644:
Page 645 and 646:
Page 647 and 648:
Page 649 and 650:
Page 651 and 652:
Page 653 and 654:
Page 655 and 656:
Page 657 and 658:
Page 659 and 660:
Page 661 and 662:
Page 663 and 664:
Page 665 and 666:
Page 667 and 668:
Page 669 and 670:
Notes - 669 -
Page 671 and 672:
- 671 -
Page 673 and 674:
- 673 -
Page 675 and 676:
Dr. Nassera Aouali L-1526 Luxembour
Page 677 and 678:
Dr. Giordano Bianchi Clinic of inte
Page 679 and 680:
Mrs. Isabel Burghardt Laboratory of
Page 681 and 682:
Pr. Czeslaw Cierniewski 92-215 Lodz
Page 683 and 684:
Mr. Igotz Delgado Biochemistry 4894
Page 685 and 686:
Mrs. Anissa Fergani INSERM U-692 67
Page 687 and 688:
Dr. Mark Ginsberg Mail code 0726 92
Page 689 and 690:
Dr. Jochen Hefl Division of Signal
Page 691 and 692:
Mr. Jan Jepsen 2100 Copenhagen DNK
Page 693 and 694:
Pr. Young Min Kim Division of Biolo
Page 695 and 696:
Mr. Christoph Lahtz AG Tumorgenetik
Page 697 and 698:
Pr. Etta Livneh 84105 Beer Sheva IS
Page 699 and 700:
Mrs. Rasa Merzvinskyte Department o
Page 701 and 702:
Mr. Gustav Nilsonne Department of L
Page 703 and 704:
Mrs. Christel Péqueux Tumour and D
Page 705 and 706:
Dr. Gabriella Regis Tumor Immunolog
Page 707 and 708:
Dr. Carsten Scheller 97078 Wuerzbur
Page 709 and 710:
Mr. Yoo-Cheol Song Laboratory of Gy
Page 711 and 712:
Dr. Lucia Maria Valatro Laboratori
Page 713 and 714:
Mrs. Jessica Wagener 40225 Duesseld
Page 715:
Pr. Moncef ZOUALI Centre Viggo Pete
show all

Visit our Expo - Redox and Inflammation signaling 2012

Create successful ePaper yourself

Delete template?

Save as template?