Visit our Expo - Redox and Inflammation signaling 2012

Session XII : Cell death and neurodegenerative diseases Poster XII, 10
Attenuation of A#-induced apoptosis of plant extract (Shaengshik) mediated by the
inhibition of mitochondrial dysfunction and anti-oxidative effect
Chu-Yue Chen 1 , Jung-Hee Jang 1 , Mi Hyun Park 2 , Sung Joo Hwang 2 , Young-Joon Surh 1 ,
Ock Jin Park 3
1 College of Pharmacy, Seoul National University, Seoul, Korea
2 Eromlife R &D center Eromlife Cooporation, Korea
3 Department of Food and Nutrition, Hannam University, 133 Ojeong-dong Daedeok-gu,
Daejeon 306-791, Korea, E-mail: ojpark@hannam.ac.kr
Recently, considerable attention has been focused on dietary manipulation of oxidative and/or
nitrosative damage on neuronal cells. In this study, a neuroprotective effect of plant
(Shaengshik) extracts was investigated. Rat pheochromocytoma (PC12), cells treated with
A#-amyloid underwent apoptotic death as determined by positive in situ terminal endlabeling
(TUNEL staining), decreased mitochondrial transmembrane potential, and elevated
caspase-3 activity co-occurring with enhanced MDA accumulation and the reduction of GSH
levels. Shaengshik pretreatment attenuated A#-amyloid-induced apoptosis in PC12 cells
possibly by inhibiting mitochondrial dysfunction and exerting antioxidant properties.
Shaengshik pretreatment inhibited the loss of mitochondrial membrane potentials and reduced
the activation of caspase-3. The in vitro antioxidant activities of Shaengshik extracts were
verified by the DPPH method and superoxide dismutase (SOD) mimetic activity. In A#amyloid-challenged
PC 12 cells, Shaengshik prevented the production of ROS, decreased the
level of MDA, and elevated GSH. The potential of Shaengshik as one of the neuroprotective
regimens has been suggested through this study, and the combination with defined
pharmaceuticals or other dietary antioxidants may provide a better therapeutic or preventive
advantage for the management of Alzheimer diseases.
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