Visit our Expo - Redox and Inflammation signaling 2012

Session XIV : Transcriptional and translational control Poster XIV, 4
Proteomic analysis for Helicobacter pylori - infected human gastric mucosa: oxidative
stress - related proteome changes
Hye Yeon BAEK, Joo Weon LIM, and Hyeyoung KIM*
Department of Pharmacology and Brain Korea 21 Project for Medical Science, Yonsei
University College of Medicine, Seoul 120-752, Korea, E-mail: ehrehrwh@hanmail.net
Helicobacter pylori (H. pylori) infection leads to gastroduodenal inflammation, peptic
ulceration and gastric carcinoma. Proteomic analysis for human gastric mucosa of the patients
with erosive gastritis or peptic ulcer, which were either infected or non-infected with H.
pylori, was used to determine the differentially expressed proteins by H. pylori in human
gastric mucosa to investigate the pathogenic mechanism of H. pylori-induced gastric diseases.
Mass spectrometry analysis (MALDI-TOF) of tryptic fragment and data search allowed
identification of increased four proteins (78kD glucose-regulated protein precursor,
endoplasmin precursor, aldehyde dehydrogenase 2, L-lactate dehydrogenase B chain) and
decreased four proteins (intracellular chloride channel protein 1, glutathione-s-transferase,
heat shock protein 60, cytokeratin 8) caused by H. pylori infection in gastric mucosa. These
proteins are related to cell proliferaton, carcinogenesis, cytoskeletal function, and cellular
defensive mechanism. Common feature is that these proteins are related to oxidative stressmediated
cell damage. In conclusion, the established gastric mucosal proteome map could be
useful for the detection of diseases-related protein changes. H. pylori-induced alterations in
protein expression demonstrates the involvement of oxidative stress in the pathogenesis of H.
pylori-induced gastric diseases including inflammation.
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