Visit our Expo - Redox and Inflammation signaling 2012

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Session XVII : Cell signaling in health and disease Poster XVII, 7 Expression and role of phosphatidylcholine-specific phospholipase C (PC-PLC) in human NK cells and its relationship with CD16 receptor. Serena Cecchetti, Francesca Spadaro, Franca Podo and Carlo Ramoni. Department of Cell Biology and Neurosciences, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161, Rome Italy. Phosphatidylcholine hydrolysis by a specific phospholipase C (PC-PLC) is a widespread response elicited by most growth factors, cytokines, neurotrasmitters, hormones and other extra-cellular signals. Our previous studies showed that PC-PLC is involved in the functional role of NK-mediated cell killing and in the lytic granules exocytosis process. PC-PLC was detected both on specific cytoplasmic compartments and on the outer membrane surface, in which the enzyme translocated upon cell activation and lytic ability maturation. Interestingly, PC-PLC expression on NK membrane surface was directly proportional to that of CD16, the low-affinity receptor for the Fc fragment of IgG, suggesting a possible correlation between the enzyme and NK cell maturation. In the present work we analyzed the trafficking from the plasma membrane to cytoplasmic regions of CD16 receptor and PC-PLC enzyme. Both proteins were internalized upon antibody engagement, degraded and newly synthetized, moreover, they needed an integral and functional actin cytoskeleton during their trafficking. Pre-incubation of NK cells with the PC- PLC inhibitor tricyclodecan-9-yl-potassium xanthate (D609) determined a dramatic decrease of PC-PLC fraction expressed on NK plasma membrane. Surprisingly, D609 also downmodulated the membrane expression of CD16 receptor. Among phenotype markers of peripheral blood lymphocytes analyzed after D609 treatment, only CD16 was deeply downmodulated, thus suggesting a possible specific role of PC-PLC enzyme in regulating CD16 receptor expression. Moreover, we demonstrated that PC-PLC was activated, within 5 minutes, upon CD16 stimulation, thus suggesting an involvement of the enzyme in CD16 signal transduction. We also evidenced that in antibody-dependent cellular cytotoxicity process PC-PLC played a double functional role both in regulating CD16 membrane expression and in transducing CD16 receptor signals, since D609 totally abolished this important lytic mechanism. - 614 -
Session XVII : Cell signaling in health and disease Poster XVII, 8 Different ways to arrest proliferation of cancer cells by dsRNA Elena L.Chernolovsksya, Tatyana O. Kabilova, Al`bina V. Vladimirova, Valentin V. Vlassov Institute of Chemical Biology and Fundamental Medicine SB RAS, Lavrentiav ave., 8, Novosibirsk 630090, Russia, E-mail: elena_ch@niboch.nsc.ru Deregulation of genes encoding components of signaling pathways are considered as a key factor in the development of different types of tumors in humans. We investigated inhibition of MYC genes by dsRNAs in KB-3-1, SC-N-MC and IMR-32 cells. Sequence-specific siRNA (si3ex) targeted to the third exon of c-myc gene was found to decrease the level of cmyc, but not N-myc mRNA. si3ex decreased the rate or even arrested the proliferation of cmyc overexpressing cell lines KB-3-1 and SC-N-MC, but did not affect the proliferation of IMR-32 (which overexpress N-myc). si2ex homological to the conservative region of the second exon of both c- and N-myc was able to downregulate both genes and to reduce proliferation of KB-3-1, SC-N-MC and IMR-32 cells. PKR or/and OAS1 mRNA levels were not effected. Long double stranded RNAs and some short dsRNA can stimulate innate cytokine responses in mammals, which can induce proliferation blockage, differentiation or apoptosis in cancer cells. Long double stranded RNAs: dsMyc homologous to the 3 exon of cmyc gene, dsGFP homologous to mRNA of EGFP gene and GU-rich siRNA (siI), homologous to the intron sequence of human MDR1 gene were found to inhibit proliferation and to decrease the mRNA level of interferon-sensitive genes: c-myc and beta-actin when delivered into cancer human cells. The level of downregulation was march higher than that of synthetic interferon inducer poly(I:C) and is likely depends on the properties of dsRNA: the thermodynamic stability, nuclease resistance and affinity to dsRNA-binding proteins. Antiproliferation activity of long dsRNA, displayed in the absence of transfection reagent, was cell line specific and correlated with the ability of dsRNA to inhibit the expression of cmyc and beta-actin genes. The elevation of PKR or/and OAS1 mRNA levels was detected in all cells affected by long dsRNA and does not correlates with proliferation blockage. The data suggest, that double stranded RNAs can serve as antiproliferative agents, acting sequencespecifically or activating innate immunity response. This work was supported by RFBR (grant No. 06-04-49480- ), RAS programs “Molecular and Cellular Biology” and “Fundamental science for medicine”, Interdisciplinary grant from SB RAS, FCSTP RI-012/001/254. - 615 -
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- 1 - Luxembourg, January 25th to 2
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Table of content PREFACE ..........
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Preface In 1998, we organized the f
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Acknowledgments This meeting has be
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Group B (15h00 - 17h00) Session V.
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- 11 - Exhibition
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Visit our Expo (in alphabetical ord
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Thursday January 26th, 2006 (Early
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Thursday January 26th, 2006 (Evenin
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Regulation of inflammation and gluc
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Saturday January 28th, 2006 (Early
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Oral presentations (by alphabetical
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4: : Lottin S, Vercoutter-Edouart A
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the B-Raf/mitogen-activated/extrace
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Albertini MC, Accorsi A, Citterio B
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AP-1-dependent gene expression in s
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Jaks and cytokine receptors - an in
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The Role of Met-Gab1 Signalling in
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The mammalian tumor suppressor Scri
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SIGNAL TRANSDUCTION BY STRESS-ACTIV
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Title to be announced Caroline Dive
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Mechanisms of DNA methylation in ma
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Blocking the "4 Integrin-Paxillin I
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Regulation of NF-kB-dependent trans
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The immunomodulator AS101 induces g
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Signaling pathways leading to the a
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Boute, N., Jockers, R. and Issad, T
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Jespsen JS, Sorensen MD and Wengel
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8. Nishikawa, H., Kawai, K., Tanaka
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9) Proteome analysis of rat pancrea
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MicroRNA is an anti-apoptotic facto
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Resveratrol inhibits phorbol ester-
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Cross-talk between angiotensin II a
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Multiple role of histamine H 1 rece
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PI3K Targeting by the Beta-GBP Cyto
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D.P. Chopra, R.E. Menard, J. Janusz
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[2] Meijer, L., Flajolet, M. and Gr
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[4] Niemand, C., Nimmesgern, A., Ha
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Oncogenic signaling by mutant p53 M
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activity of the Peroxisome Prolifer
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Restauration of SHIP-1 activity in
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Hypoxia Signaling. Impact on Tumor
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5. Sokolov EI, Zykov KA, Pukhalsky
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HOW ANTITOXIC AND ANTICANCER AGENTS
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Listeria monocytogenes induced Rac1
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Epigenetic Regulation of Stem Cell
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Survey on in vitro cell death induc
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Distinct roles of IRF-3 and IRF-7 i
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Redox-Sensitive Transcription Facto
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Epigenic control of MHC2TA transcri
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Attenuation of MSK1-driven NF-kB ge
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Talking to chromatin: Silencers Sil
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Ubiquitin ligase Smurf1 controls os
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Workshop presentations (by alphabet
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Ambion MicroRNAs (miRNAs) are small
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Organizer: BIOBASE GmbH Date: Janua
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Reliable and efficient gene Knock-d
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Exploring ubiquitin signaling with
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Poster presentations Poster are cla
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Session I : protein domains Poster
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Session II. Receptor signaling and
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Session II : Receptor signaling and
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Session III. Protein kinase cascade
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Session III : Protein kinase cascad
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Session IV. Protein kinase inhibito
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IV. Protein kinase inhibitors: insi
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IV. Protein kinase inhibitors: insi
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V. Phosphatases as key cell signali
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VI. Proteasome degradation pathways
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VI. Proteasome degradation pathways
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Session VII. Stem cell specific cel
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VIII. Inflammation specific signali
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Session IX. Novel compounds targeti
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Session X : Cell death in cancer -
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Session X : Cell death in cancer Po
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Session XI : Cell death and cardiov
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Session XI: Cell death and cardiova
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Session XII : Cell death and neurod
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Session XIII : Cell signaling pathw
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Session XIV : Transcriptional and t
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Session XVII : Cell signaling in he
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Dr. Nassera Aouali L-1526 Luxembour
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Dr. Giordano Bianchi Clinic of inte
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Mrs. Isabel Burghardt Laboratory of
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Pr. Czeslaw Cierniewski 92-215 Lodz
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Mr. Igotz Delgado Biochemistry 4894
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Mrs. Anissa Fergani INSERM U-692 67
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Dr. Mark Ginsberg Mail code 0726 92
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Dr. Jochen Hefl Division of Signal
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Mr. Jan Jepsen 2100 Copenhagen DNK
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Pr. Young Min Kim Division of Biolo
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Mr. Christoph Lahtz AG Tumorgenetik
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Pr. Etta Livneh 84105 Beer Sheva IS
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Mrs. Rasa Merzvinskyte Department o
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Mr. Gustav Nilsonne Department of L
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Mrs. Christel Péqueux Tumour and D
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Dr. Gabriella Regis Tumor Immunolog
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Pr. Moncef ZOUALI Centre Viggo Pete
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