Visit our Expo - Redox and Inflammation signaling 2012

Session X : Cell death in cancer Poster X, 41
Buddlejasaponin IV and Pleurospermum kamtschaticum extract induce apoptosis by
reducing alpha(2)beta(1) integrin-mediated adhesion in HT-29 human colon cancer cells
Jin-Eun Kim1,2,3, Won-Yoon Chung1,2, Hee-Juhn Park4, Hyun-Ju Jung4 and Kwang-
Kyun Park1,2,3
1Department of Oral Biology, 2Oral Science Research Center, College of Dentistry,
3Brain Korea 21 Project for Medical Science, Yonsei University, Seoul 120-752,
4Department of Botanical Resources, Sangji University, Wonju 220-702, E-mail:
judyjean@hanmail.net
Buddlejasaponin " (BS-") is one of the active components of
Pleurospermum kamtschaticum (Umbelliferae). The aerial part of P. kamtschaticum is used
traditionally to treat cold, atherosclerosis, arthritis and to overcome fatigue in Korea.
However, the antitumor effect of the aerial part of P. kamtschaticum extract (Pl-K) and BS-"
has not been studied. Integrins are cell surface adhesion receptors that regulate cell survival
and cell growth in response to cues derived from the extracellular matrix. Alpha(2)beta(1)
integrin downregulation induces apoptosis in various colon cancer cell lines. We investigated
whether apoptosis induced by Pl-K and BS-" was associated with alterations in integrin
signaling. Pl-K and BS-" induced apoptosis in HT-29 cells through mitochondrial dependent
pathway. Additionally, Pl-K and BS-" decreased the cell attachment to type# and "
collagen, resulting from the downregulation of alpha(2)beta(1) integrin. Furthermore, Pl-K
and BS-" inhibited expression and phosphorylation of FAK, Akt, and ERK. These results
suggest that Pl-K and BS-" induce apoptosis and inhibit survival by reducing alpha(2)beta(1)
integrin-mediated adhesion in HT-29 cells.
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