Visit our Expo - Redox and Inflammation signaling 2012

Session X : Cell death in cancer Poster X, 34
INVOLVEMENT OF TRACE ELEMENT CONTAINING-PROTEINS IN THE
APOPTOSIS AND REDOX PROCESSES IN THE PROSTATE AND HUMAN
PROSTATE CELLS
I. Grbavac, C. Wolf, N. Wenda, D. Alber, M. Kühbacher, D. Behne, A. Kyriakopoulos
Hahn-Meitner-Institut Department for molecular trace element research in the life
science Glienicker Str. 100, 14109 Berlin, Germany
It is well known that trace elements play a significant role in many functions of the human
body. Most of them are bound to proteins and have an important function like selenium and
zinc in the prostate. Trace elements like copper and zinc are known for their role in the
apoptosis of different cells. In order to obtain more information about several trace elements
in this organ, analytical methods such as instrumental neutron activation analysis (INAA) and
ICP-MS, which are appropriate procedures to determine trace elements, were used. To find
out, whether some of these elements are bound to proteins of the prostate, the gland was
homogenized and the proteins in the homogenate were separated by electrophoretic
techniques. Several antibodies against specific prostate proteins were tested. By means of
these antibodies several trace element containing-proteins such as selenoproteins and Cu- Znproteins
could be identified which are involved in the apoptosis and redox processes. By
combining of SEC and ICP-MS some trace elements, which are bound to proteins were
detected in the prostate cytosol and human prostate cell lines. The spectra of the SEC-ICP-MS
let assume that trace elements like copper, zinc, manganese and others have a strong affinity
to several proteins in the prostate. The finding of some new metals- and metalloids containing
proteins in the micro-organelles of the prostate, let assume the existence of metalloproteins
and metalloidproteins in this organ which might be play a role in the apoptosis and redox
system. In this paper some characteristics are present with regard to the newly found trace
element -containing proteins and their relationship to the redox processes.
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