Visit our Expo - Redox and Inflammation signaling 2012

Session XIV : Transcriptional and translational control Poster XIV, 47
Process simulation in a mechatronic bioreactor device with speed regulated motors for
growing of three-dimensional cell cultures
M. Mihailova1, V. Trenev2, P. Genova2, S. Konstantinov3
1IPF of TU-Sofia, 8800 Sliven, 59 Bourgasko chaussee Str, Bulgaria;
e-mail: mina-todorova@abv.bg
2CLMI, Bulgarian Academy of Sciences, 1 Acad. G. Bonchev Str, 1113 Sofia, Bulgaria;
e-mail: vtrenev@clmi.bas.bg; Pgen@TU-Sofia.bg
3Lab for Experimental Chemotherapy, Dept. of Pharmacology, Faculty of Pharmacy,
Medical University of Sofia, 1000 Sofia, 2 Dunav Str, Bulgaria;
e-mail: Konstantinov.spiromihaylov@gmail.com
Tissue engineering is a new scientific research field that allows the establishment of tissue
equivalents rising from isolated cells in combination with biocompatible materials and
cultivation in more or less sophisticated bioreactor systems. Such systems gave the unique
opportunity to perform in vitro investigations of transcription and translation, cell growth,
biochemistry and mechanics of healthy normal organs as well as those affected by malignant
tumours, infections and immune deficiency under controlled conditions. In rotating vessel
bioreactors under microgravity and defined medium content cells proliferate, stay abundant to
each other and form three-dimensional structures, assigned as spheroids. Such spheroids
might be grown on micro carriers. A wide spectrum of different cell culture experiments
involving normal and transformed human cells indicate that: in a rotating bioreactor system
miniPERM no complete lack of gravity could be reached; a great part of the seeded cell
material do not proliferate at the beginning and the appearance of bigger spheroids is rather
random. We describe the acquisition of spheroids in HD-MY-Z and Neuro-2A tumour cell
lines as well as in bone marrow fibroblasts from acute myeloid leukaemia patients. Spheroids
of 100 and more cells were obtained from HD-MY-Z and Neuro-2A cells. Interestingly,
chronic myeloid leukaemia LAMA-84 cells did not form any cell clumps and they kept a
completely undifferentiated phenotype despite their semi-adherent growth manner under
conventional conditions. A detailed theoretical and virtual simulation study of the influence of
every component of gravitation, inertia and hydrodynamic force fields was performed.
Thereby, a new concept for mechatronic bioreactor device with active electronic control was
developed and virtually tested.
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