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Session X : Cell death in cancer Poster X, 97 Effects of gamma and proton irradiation on human HTB140 melanoma cell growth, induction of apoptosis and cell cycle redistribution Danijela V. Todorovic1, Ivan M. Petrovic1, Lela B. Koricanac1, Lucia M. Valastro2, Luigi Raffaele2, Giacomo Cuttone2, Aleksandra M. Ristic-Fira1 1 Vin(a Institute of Nuclear Sciences, Belgrade, Serbia and Montenegro 2 Istituto Nazionale di Fisica Nucleare, LNS, Catania, Italy E-mail: aristic@vin.bg.ac.yu Human HTB140 melanoma cells were irradiated with either gamma rays or protons, over the dose range of 8 to 24 Gy. Cell growth was investigated using microtetrasolium (MTT) and sulforhodamine B (SRB) assays 7 days after irradiation, time needed for cell survival evaluation. With the increase of dose significant growth inhibition for both irradiation qualities was detected. The same dose level of protons induced stronger inactivation of HTB140 cells than single irradiation with gamma rays. Cell proliferation, detected by 5bromo-2 -deoxyuridine (BrdU) incorporation assay 7 days after proton and gamma irradiation, has shown highly significant (p
Session X : Cell death in cancer Poster X, 98 Inhibition of cell-free apoptosis in Xenopus oocyte extracts by Src kinase Alexander A. Tokmakov1, Mikako Shirouzu1, Shigeyuki Yokoyama1,2,3 1Genomic Sciences Center, RIKEN Yokohama Institute, Tsurumi, Yokohama, 230-0045 Japan, 2RIKEN Harima Institute at Spring-8, 3University of Tokyo, Japan. E-mail: tokmak@gsc.riken.go.jp Oncoproteins can induce both cell proliferation and apoptosis, depending on cellular context. v-Src oncoprotein has been shown to activate several kinases, which can inhibit apoptosis, including ERK and PI3-K. To further characterize the involvement of Src kinase in apoptosis, we investigated the effect of Src overexpresion in the cell-free extracts prepared from oocytes and eggs of the African clawed frog, Xenopus laevis. These extracts can recapitulate a range of apoptotic events, including release of cytochrom C, caspase activation, and nuclear fragmentation (Newmeyer et al., 1994). Notably, interphase-arrested and metaphase-arrested extracts have different susceptibility to undergo apoptosis. The MAPK pathway active in metaphase-arrested extracts was shown to protect them from apoptosis (Tashker et al., 2002). In the present study we measured the caspase 3/7 activation and cytochrom C release in apoptotic cell-free Xenopus oocyte and egg extracts, overexpressing constitutively active (v- Src) and kinase negative (Src KN) mutants of Src kinase. We found that interphase-arrested oocyte extracts were highly apoptotic. They released cytochrom C and displayed elevated levels of caspase activity after several hours’-long incubation at room temperature. Caspase activation in these extracts could be prevented by overexpression of either v-Src or Src KN protein kinase mutants. No or little effect of Src overexpression on cytochrom C release was detected. On the other hand, metaphase arrested egg extracts were resistant to apoptosis and they could not be regulated by Src kinase. Our data suggest that the inhibition of apoptosis in Xenopus oocyte extracts by Src kinase is not mediated by activation of the MAPK pathway. - 431 -
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- 1 - Luxembourg, January 25th to 2
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Table of content PREFACE ..........
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Preface In 1998, we organized the f
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Acknowledgments This meeting has be
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Group B (15h00 - 17h00) Session V.
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- 11 - Exhibition
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Visit our Expo (in alphabetical ord
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Thursday January 26th, 2006 (Early
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Thursday January 26th, 2006 (Evenin
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Regulation of inflammation and gluc
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Saturday January 28th, 2006 (Early
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Oral presentations (by alphabetical
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4: : Lottin S, Vercoutter-Edouart A
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the B-Raf/mitogen-activated/extrace
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Albertini MC, Accorsi A, Citterio B
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AP-1-dependent gene expression in s
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Jaks and cytokine receptors - an in
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The Role of Met-Gab1 Signalling in
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The mammalian tumor suppressor Scri
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SIGNAL TRANSDUCTION BY STRESS-ACTIV
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Title to be announced Caroline Dive
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Mechanisms of DNA methylation in ma
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Blocking the "4 Integrin-Paxillin I
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Regulation of NF-kB-dependent trans
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The immunomodulator AS101 induces g
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Signaling pathways leading to the a
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Boute, N., Jockers, R. and Issad, T
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Jespsen JS, Sorensen MD and Wengel
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8. Nishikawa, H., Kawai, K., Tanaka
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9) Proteome analysis of rat pancrea
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MicroRNA is an anti-apoptotic facto
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Resveratrol inhibits phorbol ester-
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Cross-talk between angiotensin II a
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Multiple role of histamine H 1 rece
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PI3K Targeting by the Beta-GBP Cyto
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D.P. Chopra, R.E. Menard, J. Janusz
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[2] Meijer, L., Flajolet, M. and Gr
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[4] Niemand, C., Nimmesgern, A., Ha
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Oncogenic signaling by mutant p53 M
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activity of the Peroxisome Prolifer
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Restauration of SHIP-1 activity in
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Hypoxia Signaling. Impact on Tumor
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5. Sokolov EI, Zykov KA, Pukhalsky
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HOW ANTITOXIC AND ANTICANCER AGENTS
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Listeria monocytogenes induced Rac1
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Epigenetic Regulation of Stem Cell
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Survey on in vitro cell death induc
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Distinct roles of IRF-3 and IRF-7 i
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Redox-Sensitive Transcription Facto
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Epigenic control of MHC2TA transcri
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Attenuation of MSK1-driven NF-kB ge
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Talking to chromatin: Silencers Sil
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Ubiquitin ligase Smurf1 controls os
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Workshop presentations (by alphabet
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Ambion MicroRNAs (miRNAs) are small
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Organizer: BIOBASE GmbH Date: Janua
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Reliable and efficient gene Knock-d
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Exploring ubiquitin signaling with
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Poster presentations Poster are cla
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Session I : protein domains Poster
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Session II. Receptor signaling and
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Session II : Receptor signaling and
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Session III. Protein kinase cascade
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Session III : Protein kinase cascad
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Session IV. Protein kinase inhibito
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IV. Protein kinase inhibitors: insi
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IV. Protein kinase inhibitors: insi
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V. Phosphatases as key cell signali
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VI. Proteasome degradation pathways
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VI. Proteasome degradation pathways
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Session VII. Stem cell specific cel
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VIII. Inflammation specific signali
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Session IX. Novel compounds targeti
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Session X : Cell death in cancer -
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Session X : Cell death in cancer Po
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Session XII : Cell death and neurod
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Session XII : Cell death and neurod
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Session XIII : Cell signaling pathw
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Session XIV : Transcriptional and t
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Session XV : Reactive oxygen specie
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Session XVI : Chemopreventive agent
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Session XVII : Cell signaling in he
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Dr. Nassera Aouali L-1526 Luxembour
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Dr. Giordano Bianchi Clinic of inte
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Mrs. Isabel Burghardt Laboratory of
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Pr. Czeslaw Cierniewski 92-215 Lodz
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Mr. Igotz Delgado Biochemistry 4894
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Mrs. Anissa Fergani INSERM U-692 67
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Dr. Mark Ginsberg Mail code 0726 92
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Dr. Jochen Hefl Division of Signal
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Mr. Jan Jepsen 2100 Copenhagen DNK
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Pr. Young Min Kim Division of Biolo
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Mr. Christoph Lahtz AG Tumorgenetik
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Pr. Etta Livneh 84105 Beer Sheva IS
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Mrs. Rasa Merzvinskyte Department o
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Mr. Gustav Nilsonne Department of L
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Mrs. Christel Péqueux Tumour and D
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Dr. Gabriella Regis Tumor Immunolog
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Mr. Yoo-Cheol Song Laboratory of Gy
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Pr. Moncef ZOUALI Centre Viggo Pete
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