Visit our Expo - Redox and Inflammation signaling 2012

Crosstalk between NF-kB and AP-1 in Helicobacter pylori-mediated inflammation in
human gastric epithelial cells
Hyeyoung Kim
Department of Pharmacology and Brain Korea 21 Project for Medical Science, Yonsei
University College of Medicine, Seoul 120-752, Korea. Email:
kim626@yumc.yonsei.ac.kr
Previously we have shown that inflammatory enzymes such as cyclooxygenase-2 (COX-2)
and inducible nitric oxide syntahse (iNOS) were expressed in H. pylori-infected gastric
epithelial cells. The expression of COX-2 and iNOS may be regulated by NF-kB and AP-1.
Present study aims to investigate whether H. pylori-induced expressions of COX-2 and iNOS
are regulated by NF-kB and AP-1 in gastric epithelial AGS cells, and whether the
transcriptional regulation of COX-2 or iNOS is inhibited by transfection of antisense
oligodeoxynucleotide (AS ODN) for NF-kB subunit p50 or c-jun dominant negative mutant
gene (TAM-67). As a result, H. pylori induced the expression of COX-2 and iNOS via
activation of NF-kB and AP-1, which was inhibited in the cells transfected with either p50 AS
ODN or TAM-67. The activated NF-kB complex was p65/p50 hetero- and p50/p50 homo-
dimmers while the activated AP-1 complex was a c-jun/c-fos heterodimer in H. pyloriinfected
AGS cells. In conclusion, NF-kB and AP-1 share H. pylori-mediated inflammation
signaling by inducing COX-2 and iNOS expression in gastric epithelial cells. Either
suppression of NF-kB or AP-1 may inhibit H. pylori-induced inflammation in gastric
epithelial cells.
Publication list (2002-2005)
1) NADPH oxidase and apoptosis in cerulein-stimulated pancreatic acinar cells. JH Yu, JW
Lim, KH Kim, T Morio, H Kim. Free Rad Biol Med 39: 590-602, 2005
2) NADPH oxidase mediates interleukin-6 expression in cerulein-stimulated pancreatic acinar
cells. JH Yu, JW Lim, H Kim, KH Kim, Int J Biochem Cell Biol 37:1458-1469, 2005
3) Cellular stress - related protein expression in Helicobacter pylori - infected gastric
epithelial AGS cells. JW Lim, H Kim, JM Kim, JS Kim, HC Jung, KH Kim, Int J Biochem
Cell Biol 36:1624-1634, 2004.
4) Oxidative stress - related proteome changes in Helicobacter pylori - infected human gastric
mucosa. HY Baek, JW Lim, H Kim, JM Kim, JS Kim, HC Jung, KH Kim, Biochem J
379:291-299, 2004
5) Helicobacter pylori in a Korean isolate activates mitogen-activated protein kinases, AP-1,
and NF-!B and induces chemokine expression in gastric epithelial AGS cells. JH Seo, JW
Lim, H Kim, KH Kim, Lab Inv 84:49-62, 2004
6) The Ku antigen-recombination signal-binding protein J! complex binds to the nuclear
factor-!B p50 promoter and acts as a positive regulator of p50 expression in human gastric
cancer cells. JW Lim, H Kim, KH Kim, J Biol Chem 279(1):231-237, 2004
7) Role of oxygen free radicals in patients with acute pancreatitis. BK Park, JB Chung, JH
Lee, JH Suh, SW Park, SY Song, H Kim, KH Kim, JK Kang, World J Gastroenterology
9(10):2266-2269, 2003
8) Oxidative stress induces nuclear loss of DNA repair proteins Ku70 and Ku80 and apoptosis
in pancreatic acinar AR42J cells. JY Song, JW Lim, H Kim, T Morio, KH Kim.. J Biol Chem
278 (38):36676-36687, 2003 Oct.
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