Visit our Expo - Redox and Inflammation signaling 2012

Session XIV : Transcriptional and translational control Poster XIV, 41
Role of VEGF-D and AT2 receptor on cytokine expression in pancreatic acinar cells
JANGWON LEE, SHIN YOUNG YUN, JOO WEON LIM, KYUNG HWAN KIM, and
HYEYOUNG KIM
Department of Pharmacology, Brain Korea 21 Project for Medical Science, Yonsei
University College of Medicine, Seoul 120-752, Korea. E-mail :honorjw@hotmail.com
Cerulein pancreatitis is similar to human edematous pancreatitis with dysregulation of the
digestive enzyme production, the death of acinar cells, and an infiltration of inflammatory
cells into the pancreas. Our previous proteomic analysis showed several differentially
expressed proteins in cerulein-treated pancreatic acinar cells, which are related to cellular
stress such as reactive oxygen species. We hypothesized that membrane proteins may be
altered as the early event during the induction of acute pancreatitis. The present study was
designed to elucidate firstly whether or not VEGF receptor and AT2 receptor be involved in
controlling cytokine expression. Secondly, the role of VEGF-D and AT2 receptor in
pancreatic acinar cells and their regulation by acute pancreatitis are also investigated. The
differentially expressed membrane proteins by cerulein will provide valuable information to
understand pathophysiologic mechanism of acute pancreatitis and may be useful for
prognostic indices of acute pancreatitis. AP-1 and NF-kB regulate cytokine gene expression
in pancreatic acinar cells treated with cerulein. AT2 receptor is associated with VEGF-D to
induce IL-6 expression in AR42J cells. Cerulein-induced VEGF-D expression and AP-1/NFkB
activation were inhibited in the cells transfected with VEGF-D antisense oligonucleotide,
indicating the involvement of VEGF-D in regulating the cytokine expression during acute
pancreatitis.
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