Visit our Expo - Redox and Inflammation signaling 2012

Session XVI : Chemopreventive agents Poster XVI, 10
Resveratrol inhibits the growth of PA-1 ovarian cancer cells by down-regulating
eEF1A2 expression
Mee-Hyun Lee1, Bu-Young Choi2 and Young-Joon Surh1
1National Research Laboratory of Molecular Carcinogenesis and Chemoprevention,
College of Pharmacy, Seoul National University, Seoul 151-742 and 2C&C Research
Labs, Hwasung City, Gyeonggi-do 445-970, South Korea
The eukaryotic elongation factor 1A2 (eEF1A2), a subtype of eEF1A that is a key factor in
the translational process of protein synthesis, promotes the transfer of animoacylated tRNAs
to the A site of the ribosome. In human, eEF1A2 expression is restricted to the heart, brain,
and skeletal muscle. Recently, eEF1A2 has been shown to be a potential oncogene that is
over-expressed in ovarian cancer. Therefore, the regulation of inappropriate expression of
eEF1A2 is considered as a rational strategy for ovarian cancer therapy. Resveratrol (3,4’,5trihydroxy
stilbene), a phytoalexin present in grapes, has been reported to possess antioxidant,
anti-inflammatory, and anti-carcinogenic activities. In the present study, we examined the
anti-carcinogenic effect of resveratrol in PA-1 ovarian cancer cells considering eEF1A2 as a
potential target. Resveratrol inhibited the growth of PA-1 cells. Treatment of PA-1 cells with
insulin (10 µg/ml) or FBS (1%) resulted in the induction of eEF1A2. Pretreatment of PA-1
cells with resveratrol suppressed insulin- or FBS-induced expression of eEF1A2. Previous
studies have demonstrated that eEF1A2 confers anti-apoptotic effect by down-regulating
caspase-3 activity. Resveratrol was found to induce caspase-3 activity and cause apoptosis in
eEF1A2 over-expressing cells, suggesting that the induction of apoptosis by resveratrol in
PA-1 cells is mediated, at least in part, via down-regulation of eEF1A2 expression. Taken
together, these results suggest that oncogenic eEF1A2 is a potential target for ovarian cancer
chemoprevention with resveratrol.
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