Visit our Expo - Redox and Inflammation signaling 2012

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Mechanisms involved in the anti-apoptotic effect of magnetic fields Maria C. Albertini, Claudia Cerella *, Sonia Cordisco*, Augusto Accorsi, Antonio Bergamaschi#, Maria D'Alessio*, Milena De Nicola*, Silvia Nuccitelli*, Lina Ghibelli* Istituto di Chimica Biologica A. Fornaini, Universita' di Urbino, Urbino, Italy *Dipartimento di Biologia, #Cattedra di Medicina del Lavoro, Universita' di Roma Tor Vergata, Rome, Italy 00133; *. E-mail: cemetbio@uniurb.it Concern for possible health hazard due to increasing exposure to magnetic fields (MFs), came from epidemiological reports indicating a correlation between exposure to MFs and development of some types of tumors. This led to great interest in the interaction of MFs with living matter, especially focusing on possible interference on cell metabolism that may account for a pro-tumoral role of MFs. Research on this issue is made quite difficult since different types of MFs (i.e., static vs oscillating; different oscillation frequencies; different intensities; different vector directions; etc.) may differently interact with cells, thus giving rise to controversial results. We showed that 0.3-60 mTesla (mT) static (i.e., magnete-induced) MFs may well act as tumor promoting agents, tumor promotion being the epigenetic effect that allows tumor growth by favoring the growth/survival of transformed cells. Indeed, MFs strongly reduce the extent of damage-induced apoptosis on a set of cell lines, thus increasing survival of possibly transformed cells (Fanelli et al., FASEB J 1999, 133:95-102). We describe here the events triggered by exposure to MFs on a reporter cell line (U937), which lead to impairment of damage-induced apoptosis, compared to an insensitve one (Jurkat). We show that MFs anti-apoptotic effect requires a correct redox equilibrium, a working phospholipase C (PLC, a key actor of receptor-mediated signal transduction), and nitric oxide syntase (NOS, a key actor of intracellular inflammatory signal transduction), the antiapoptotic effect being abolished in the presence of reducing (DTT) or oxidizing (BSO) agents; of PLC inhibitors (neomycin and U73122); and NOS inhibitors (L-NAME), respectively. Accordingly, the sensitive cells show increased Ca2+ influx, increased IP3 production, plasma membrane hyperpolarization and alterations of free radicals and glutathione levels. Jurkat cells may be rendered sensitive upon activation with phytohemagglutinin; the normal/activated Jurkat provide an ideal system where to look for the primary determinants for the sensitivity to MFs anti-apoptotic effect. Intriguingly, extremely low frequency (50Hz) electroMFs (ELF) exert similar effects, reducing apoptosis in a Ca2+ dependent fashion. LIST OF RECENT PAPERS Piacentini MP, Piatti E, Fraternale D, Ricci D, Albertini MC, Accorsi A. Phospholipase C-dependent phosphoinositide breakdown induced by ELF-EMF in Peganum harmala calli. Biochimie. 2004 Apr-May;86(4-5):343-9. Ghibelli L, Teodori L, Cerella C, De Nicola M, D'Alessio M, Clavarino G, Cordisco S, Albertini MC, Accorsi A, Magrini A, Bergamaschi A. Epigenetic role of magnetic field exposure in tumor progression: fine-tuning experimental models] G Ital Med Lav Ergon. 2003 Jul-Sep;25 Suppl(3):277-8. - 28 -
Albertini MC, Accorsi A, Citterio B, Burattini S, Piacentini MP, Uguccioni F, Piatti E. Morphological and biochemical modifications induced by a static magnetic field on Fusarium culmorum. Biochimie. 2003 Oct;85(10):963-70. Albertini MC, Teodori L, Piatti E, Piacentini MP, Accorsi A, Rocchi MB. Automated analysis of morphometric parameters for accurate definition of erythrocyte cell shape. Cytometry A. 2003 Mar;52(1):12-8. Piatti E, Albertini MC, Baffone W, Fraternale D, Citterio B, Piacentini MP, Dacha M, Vetrano F, Accorsi A. Antibacterial effect of a magnetic field on Serratia marcescens and related virulence to Hordeum vulgare and Rubus fruticosus callus cells. Comp Biochem Physiol B Biochem Mol Biol. 2002 Jun;132(2):359-65. Colussi C, Albertini MC, Coppola S, Rovidati S, Galli F, Ghibelli L. H2O2-induced block of glycolysis as an active ADP-ribosylation reaction protecting cells from apoptosis. FASEB J. 2000 Nov;14(14):2266-76. - 29 -
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Page 3 and 4: Table of content PREFACE ..........
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activity of the Peroxisome Prolifer
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Restauration of SHIP-1 activity in
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Hypoxia Signaling. Impact on Tumor
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HOW ANTITOXIC AND ANTICANCER AGENTS
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Listeria monocytogenes induced Rac1
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Epigenetic Regulation of Stem Cell
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Survey on in vitro cell death induc
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Distinct roles of IRF-3 and IRF-7 i
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Redox-Sensitive Transcription Facto
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Epigenic control of MHC2TA transcri
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Attenuation of MSK1-driven NF-kB ge
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Talking to chromatin: Silencers Sil
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Ubiquitin ligase Smurf1 controls os
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Ambion MicroRNAs (miRNAs) are small
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Organizer: BIOBASE GmbH Date: Janua
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Reliable and efficient gene Knock-d
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Exploring ubiquitin signaling with
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Session I : protein domains Poster
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Session II. Receptor signaling and
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VI. Proteasome degradation pathways
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VI. Proteasome degradation pathways
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Session XVI : Chemopreventive agent
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Dr. Nassera Aouali L-1526 Luxembour
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Dr. Giordano Bianchi Clinic of inte
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Mrs. Isabel Burghardt Laboratory of
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Pr. Czeslaw Cierniewski 92-215 Lodz
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Mr. Igotz Delgado Biochemistry 4894
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Dr. Jochen Hefl Division of Signal
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